A Comparative Study of Four Methods for Detecting Giardia Lamblia in Children With Diarrheal Disease and Malabsorption

      This paper is only available as a PDF. To read, Please Download here.
      Four methods for detecting Giardia lamblia, namely examinations of stools, duodenal aspirates, intestinal biopsies, and impression smears of intestinal mucosa, were evaluated prospectively in 21 Malaysian children with acute or chronic diarrheal disease or with malabsorption. The parasites were detected by one or more of these methods in 12 patients. Although stool examinations were diagnostic in only 6 patients, duodenal aspirates were positive in 10. All 12 infections could have been detected if results of examination of stools and duodenal aspirates were combined. Mucosal impression smears and biopsies gave the highest yield of positive tests; they were diagnostic in all 12 patients in whom parasites were detected by any of the diagnostic methods. Detection of parasites in biopsies was a tedious and time-consuming process, requiring careful scrutiny of several sections before positive identifications could be made. In contrast, evaluation of mucosal impression smears for parasites was easier and quicker than examining biopsies. Examination of mucosal impression smears for G. lamblia is recommended as a useful initial step in the routine evaluation of intestinal biopsies.
      To read this article in full you will need to make a payment
      AGA Member Login
      Login with your AGA username and password.
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Purchase one-time access:

      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Indian Practitioner
        Special number on amoebiasis and giardiasis.
        Indian Practit. 1968; 21: 613-833
        • Rendtorff RC
        Experimental transmission of human intestinal protozoan parasites. IV: Attempts to transmit Entamoeba coli and Giardia lamblia cysts by water.
        Am J Hyg. 1954; 60: 327-338
        • Hoskins LC
        • Winawer SJ
        • Broitman SA
        • et al.
        Clinical giardiasis and intestinal malabsorption.
        Gastroenterology,. 1967; 53: 265-279
        • Ament ME
        Diagnosis and treatment of giardiasis.
        J Pediatr. 1972; 80: 633-637
        • Barbezat GO
        • Bowie MD
        • Raschula ROC
        • et al.
        Studies on the small intestinal mucosa of children with protein-calorie malnutrition.
        S Afr Med J. 1967; 41: 1031-1034
        • Brandborg LL
        • Tankersley CB
        • Gottlieb S
        • et al.
        Histological demonstration of mucosal invasion by Giardia lamblia in man.
        Gastroenterology. 1967; 52: 143-150
        • Ament ME
        • Rubin CE
        The relation of giardiasis to abnormal intestinal structure and function in gastrointestinal immuno-deficiency syndromes.
        Gastroenterology. 1972; 62: 216-226
        • Ridley DS
        • Hawgood BC
        The value of formolether concentration of faecal cysts and ova.
        J Clin Pathol. 1956; 9: 74-76
        • Read AE; Gough KR
        • Bones JA
        • et al.
        An improvement to the Crosby Peroral Intestinal Biopsy Capsule.
        Lancet. 1962; 1: 894-895
        • Wicks T
        • Clain D
        A guide wire for rapid jejunal biopsies with the Crosby capsule.
        Gut. 1972; 13: 571
        • Walker-Smith JA
        Dissecting microscopic appearance of small bowel mucosa in childhood.
        Arch Dis Child. 1967; 42: 626-630
        • Rubin CE
        • Brandborg LL
        • Phelps DC
        • et al.
        Studies of coeliac disease.
        I. Gastroenterology. 1960; 38: 28-49
        • Yardley JH
        • Bayless TM
        Gastroenterology. 1967; 52: 301-303
        • Barbieri D
        • DeBrito T
        • Hoshino S
        • et al.
        Giardiasis in childhood. Absorption tests and biochemistry, histo-chemistry, light, and electron-microscopy of jejunal mucosa.
        Arch Dis Child. 1970; 45: 466-472