Molecular Diagnosis for Helicobacter pylori . . . at Last

      See “Comparison of culture with antibiogram to next-generation sequencing using bacterial isolates and formalin-fixed paraffin-embedded gastric biopsies,” by Hulten KG, Genta RM, Kalfus IN, et al, on page 1433.
      To read this article in full you will need to make a payment
      AGA Member Login
      Login with your AGA username and password.
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Bazzoli F.
        • Zagari R.
        • Fossi S.
        • et al.
        Efficacy and tolerability of a short-term low-dose triple therapy for eradication of Helicobacter pylori.
        Gastroenterology. 1993; 40A
        • Lamouliatte H.
        • Cayla R.
        • Mégraud F.
        • et al.
        Amoxicillin-clarithromycin-omeprazole: the best therapy for H. pylori infection.
        Acta Gastro-Enterologia Belgica. 1993; 56: 140
        • Lind T.
        • Veldhuyzen van Zanten S.
        • Unge P.
        • et al.
        Eradication of Helicobacter pylori using one-week triple therapies combining omeprazole with two antimicrobials: the MACH I Study.
        Helicobacter. 1996; 1: 138-144
        • Lind T.
        • Mégraud F.
        • Unge P.
        • et al.
        The MACH2 study: Role of omeprazole in eradication of Helicobacter pylori with 1-week triple therapies.
        Gastroenterology. 1999; 116: 248-253
        • Matysiak-Budnik T.
        • Heyman M.
        • Candalh C.
        • et al.
        In vitro transfer of clarithromycin and amoxicillin across the epithelial barrier: effect of Helicobacter pylori.
        J Antimicrob Chemother. 2002; 50: 865-872
        • Lee G.C.
        • Reveles K.R.
        • Attridge R.T.
        • et al.
        Outpatient antibiotic prescribing in the United States: 2000 to 2010.
        BMC Med. 2014; 12: 96
        • Shlaes D.M.
        • Gerding D.N.
        • John J.F.
        • et al.
        Society for Healthcare Epidemiology of America and Infectious Diseases Society of America Joint Committee on the Prevention of Antimicrobial Resistance: guidelines for the prevention of antimicrobial resistance in hospitals.
        Infect Control Hosp Epidemiol. 1997; 18: 275-291
        • Chen Y.
        • Zhou J.
        • Wang L.
        Role and mechanism of gut microbiota in human disease.
        Front Cell Infect Microbiol. 2021; 11: 625913
        • Chisholm S.A.
        • Owen R.J.
        • Teare E.L.
        • et al.
        PCR-based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples.
        J Clin Microbiol. 2001; 39: 1217-1220
        • Oleastro M.
        • Ménard A.
        • Santos A.
        • et al.
        Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori.
        J Clin Microbiol. 2003; 41: 397-402
        • Schabereiter-Gurtner C.
        • Hirschl A.M.
        • Dragosics B.
        • et al.
        Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens.
        J Clin Microbiol. 2004; 42: 4512-4518
        • Bénéjat L.
        • Ducournau A.
        • Lehours P.
        • et al.
        Real-time PCR for Helicobacter pylori diagnosis. The best tools available.
        Helicobacter. 2018; 23e12512
        • Jehanne Q.
        • Bénéjat L.
        • Mégraud F.
        • et al.
        Evaluation of the AllplexTM H pylori and ClariR PCR Assay for Helicobacter pylori detection on gastric biopsies.
        Helicobacter. 2020; 25e12702
        • Puz S.
        • Innerhofer A.
        • Ramharter M.
        • et al.
        A novel noninvasive genotyping method of Helicobacter pylori using stool specimens.
        Gastroenterology. 2008; 135: 1543-1551
        • Talarico S.
        • Safaeian M.
        • Gonzalez P.
        • et al.
        Quantitative detection and genotyping of Helicobacter pylori from stool using droplet digital PCR reveals variation in bacterial loads that correlates with cagA virulence gene carriage.
        Helicobacter. 2016; 21: 325-333
        • Hulten K.G.
        • Genta R.M.
        • Kalfus I.N.
        • et al.
        Comparison of culture with antibiogram to next-generation sequencing using bacterial isolates and formalin-fixed paraffin-embedded gastric biopsies.
        Gastroenterology. 2021; 161 (1433–1442)
        • Saracino I.M.
        • Pavoni M.
        • Zullo A.
        • et al.
        Next Generation Sequencing for the prediction of the antibiotic resistance in Helicobacter pylori: a literature review.
        Antibiotics (Basel). 2021; 10: 437
        • Sugano K.
        • Tack J.
        • Kuipers E.J.
        • et al.
        Kyoto global consensus report on Helicobacter pylori gastritis.
        Gut. 2015; 64: 1353-1367
        • Council of the European Union
        Council recommendation of 15 November 2001 on the prudent use of antimicrobial agents in human medicine (2002/77/EC).
        Official Journal of the European Communities. 2002; : 13-16

      Linked Article