Advertisement

Detection of Circulating Pancreas Epithelial Cells in Patients With Pancreatic Cystic Lesions

  • Andrew D. Rhim
    Correspondence
    Reprint requests Address requests for reprints to: Andrew D. Rhim, MD, Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher Place, BSRB 2061, Ann Arbor, Michigan 48108. fax: (734) 763-2162.
    Affiliations
    Division of Gastroenterology, Department of Internal Medicine, Pancreatic Cancer Center, University of Michigan Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan

    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Fredrik I. Thege
    Affiliations
    Department of Biomedical Engineering, Cornell University, Ithaca, New York
    Search for articles by this author
  • Steven M. Santana
    Affiliations
    Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, New York
    Search for articles by this author
  • Timothy B. Lannin
    Affiliations
    Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, New York
    Search for articles by this author
  • Trisha N. Saha
    Affiliations
    Division of Gastroenterology, Department of Internal Medicine, Pancreatic Cancer Center, University of Michigan Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan

    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Shannon Tsai
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Lara R. Maggs
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Michael L. Kochman
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Gregory G. Ginsberg
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • John G. Lieb
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Vinay Chandrasekhara
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Jeffrey A. Drebin
    Affiliations
    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Department of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Nuzhat Ahmad
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Yu–Xiao Yang
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
  • Brian J. Kirby
    Correspondence
    Brian J. Kirby, PhD, Sibley School of Mechanical and Aerospace Engineering, Cornell University, 238 Upson Hall, Ithaca, New York 14853. fax: (607) 255-1222.
    Affiliations
    Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, New York

    Department of Medicine, Division of Hematology/Medical Oncology, Weill Cornell Medical College, New York, New York
    Search for articles by this author
  • Ben Z. Stanger
    Correspondence
    Ben Z. Stanger, MD, PhD, Division of Gastroenterology, Department of Medicine, University of Pennsylvania 421 Curie Boulevard, 512 BRB II/III, Philadelphia, Pennsylvania 19104. fax: (215) 573-2486.
    Affiliations
    Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for articles by this author
Published:December 16, 2013DOI:https://doi.org/10.1053/j.gastro.2013.12.007
      Hematogenous dissemination is thought to be a late event in cancer progression. We recently showed in a genetic model of pancreatic ductal adenocarcinoma that pancreas cells can be detected in the bloodstream before tumor formation. To confirm these findings in humans, we used microfluidic geometrically enhanced differential immunocapture to detect circulating pancreas epithelial cells in patient blood samples. We captured more than 3 circulating pancreas epithelial cells/mL in 7 of 21 (33%) patients with cystic lesions and no clinical diagnosis of cancer (Sendai criteria negative), 8 of 11 (73%) with pancreatic ductal adenocarcinoma, and in 0 of 19 patients without cysts or cancer (controls). These findings indicate that cancer cells are present in the circulation of patients before tumors are detected, which might be used in risk assessment.

      Keywords

      Abbreviations used in this paper:

      CEC (circulating epithelial cell), DAPI (4′,6-diamidino-2-phenylindole), GEDI (geometrically enhanced differential immunocapture), IPMN (intraductal papillary mucinous neoplasm), PDAC (pancreatic ductal adenocarcinoma), Pdx-1 (pancreatic and duodenal homeobox protein-1.), CK (cytokeratin 19)
      To read this article in full you will need to make a payment
      AGA Member Login
      Login with your AGA username and password.

      Purchase one-time access:

      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Weinberg R.A.
        Carcinogenesis. 2008; 29: 1092-1095
        • Sakorafas G.H.
        • et al.
        Dig Liver Dis. 2003; 35: 482-485
        • Agarwal B.
        • et al.
        Pancreas. 2008; 36: e15-e20
        • Rhim A.D.
        • et al.
        Cell. 2012; 148: 349-361
        • Tanaka M.
        • et al.
        Pancreatology. 2006; 6: 17-32
        • Gleghorn J.P.
        • et al.
        Lab Chip. 2010; 10: 27-29
        • Kirby B.J.
        • et al.
        PLoS One. 2012; 7: e35976
        • Fritz S.
        • et al.
        Ann Surg. 2012; 256: 313-320
        • Yu M.
        • et al.
        Nature. 2012; 487: 510-513