Using Salmonella enterica Serotype Typhimurium to Model Intestinal Fibrosis

  • Beth A. McCormick
    Address requests for reprints to: Beth A. McCormick, PhD, Department of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital and Harvard Medical School, 114 16th Street (114-3503), Charlestown, Massachusetts 02129. fax: (617) 726-4172.
    Department of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts
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Published:February 13, 2008DOI:
      See “Chronic enteric Salmonella infection in mice leads to severe and persistent intestinal fibrosis” by Grassl GA, Valdez Y, Bergstrom KSB, et al on page 768.
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        • Becker J.M.
        Surgical therapy for ulcerative colitis and Crohn’s disease.
        Gastroenterol Clin North Am. 1999; 28: 371-390
        • Graham M.F.
        Pathogenesis of intestinal strictures in Crohn’s disease: an update.
        Inflamm Bowel Dis. 1995; 1: 220-227
        • Burke J.P.
        • Muslow J.J.
        • O’Keane C.
        • et al.
        Fibrogenesis in Crohn’s disease.
        Am J Gastroenterol. 2007; 102: 439-448
        • Pucilowska J.
        • Williams K.L.
        • Lund P.K.
        Fibrogenesis IV.
        Am J Physiol Gastrointest Liver Physiol. 2000; 279: G653-G659
        • Geboes K.P.
        • Cabooter L.
        • Geboes K.
        Contribution of morphology for the comprehension of mechanisms of fibrosis in inflammatory enterocolitis.
        Acta Gastroenterol Belg. 2000; 63: 371-376
        • Assche G.V.
        Can we influence fibrosis in Crohn’s disease?.
        Acta Gastroenterol Belg. 2001; 64: 193-196
        • Schuooan D.
        • Hahn E.G.
        MMPs in the gut: inflammation hits the matrix.
        Gut. 2000; 47: 12-14
        • Pucilowska J.B.
        • McNaughton K.K.
        • Mohapatra N.K.
        • et al.
        IGF-1 and procollagen alpha 1 are coexpressed in a subset of mesenchymal cells in active Crohn’s disease.
        Am J Physiol Gastrointest Liver Physiol. 2000; 279: G1307-G1322
        • Matthes H.
        • Herbst H.
        • Schuppan D.
        • et al.
        Cellular localization of procollagen gene transcripts in inflammatory bowel diseases.
        Gastroenterology. 1992; 102: 431-442
        • Rutgeerts P.
        • Geboes K.
        • Vantrappen G.
        • et al.
        Natural history of recurrent Crohn’s disease at the ileocolonic anastomosis after curative surgery.
        Gut. 1984; 25: 665-672
        • Rutgeerts P.
        • Geboes K.
        • Vantrappen G.
        • et al.
        Predictability of the postoperative cause of Crohn’s disease.
        Gastroenterology. 1990; 99: 956-963
        • Whelan G.
        • Farmer R.G.
        • Fazio V.W.
        • et al.
        Recurrence after surgery in Crohn’s disease.
        Gastroenterology. 1985; 88: 1826-1833
        • Dietz D.W.
        • Laurerti S.
        • Strong S.A.
        • et al.
        Safety and long term efficacy of strictureplasty in 314 patients with obstructing small bowel Crohn’s disease.
        J Am Cell Surg. 2001; 192: 330-337
        • McKaig B.C.
        • Hughes K.
        • Tighe P.J.
        • et al.
        Differential expression of TGF-beta isoforms by normal and inflammatory bowel disease intestinal myofibroblasts.
        Am J Physiol Cell Physiol. 2002; 282: C172-C182
        • diMola F.F.
        • Friess H.
        • Scheuren A.
        • et al.
        Transforming growth factor-betas and their signaling receptors are coexpressed in Crohn’s disease.
        Ann Surg. 1999; 229: 67-75
        • diMola F.F.
        • DiSebastiano P.
        • Gardini A.
        • et al.
        Differential expression of connective tissue growth factor in inflammatory bowel disease.
        Digestion. 2004; 69: 245-253
        • van Tol E.A.
        • Holt L.
        • Li Fl.
        • et al.
        Bacterial cell wall polymers promote intestinal fibrosis by direct stimulation of myofibroblasts.
        Am J Physiol Gastrointest Liver Physiol. 1999; 227: G245-G255
        • Moris G.P.
        • Beck P.L.
        • Herridge M.J.
        • et al.
        Hapten-induced model of chronic inflammation and ulceration in the rat colon.
        Gastroenterology. 2003; 125: 1750-1761
        • Lawrance I.C.
        • Wu F.
        • Leite A.Z.
        • et al.
        A murine model of chronic inflammation-induced intestinal fibrosis down-regulated by antisense NF-kappa B.
        Gastroenterology. 2003; 125: 1750-1761
        • Vallance B.A.
        • Gunawan M.I.
        • Hewlett B.
        • et al.
        TGF-beta1 gene transfer to the mouse colon leads to intestinal fibrosis.
        Am J Physiol Gastrointest Liver Physiol. 2005; 289: G116-G128
        • Motomura Y.
        • Khan W.I.
        • El-Sharkawy R.T.
        • et al.
        Induction of a fibrogenic response in mouse colon by overexpression of monocyte chemoattractant protein-1.
        Gut. 2006; 55: 662-670
        • Grassl G.A.
        • Valdez Y.
        • Bergstrom K.S.B.
        • et al.
        Chronic enteric Salmonella infection in mice leads to severe and persistent intestinal fibrosis.
        Gastroenterology. 2008; 134: 768-780

      Linked Article

      • Chronic Enteric Salmonella Infection in Mice Leads to Severe and Persistent Intestinal Fibrosis
        GastroenterologyVol. 134Issue 3
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          Background & Aims: Intestinal fibrosis and stricture formation are serious complications of Crohn’s disease, often requiring surgical intervention. Unfortunately, the mechanisms underlying intestinal fibrosis development are poorly understood, in part because of the lack of relevant animal models. Here, we present a novel murine model of severe and persistent intestinal fibrosis caused by chronic bacterial-induced colitis. Methods: Mice were treated with streptomycin 24 hours prior to oral infection with Salmonella enterica serovar Typhimurium.
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