Gastroenterology
Volume 139, Issue 3 , Pages 1019-1029 , September 2010

Therapeutic Liver Reconstitution With Murine Cells Isolated Long After Death

  • Laura Erker

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • Corresponding Author InformationReprint requests Address requests for reprints to: Laura Erker, PhD, Oregon Stem Cell Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, L-321, Portland, Oregon 97239. fax: (503) 418-5044
    • ,
  • Hisaya Azuma

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • ,
  • Andrew Y. Lee

      Affiliations

    • Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California
    • Department of Surgery, Division of Transplantation, University of California, San Francisco, California
    • ,
  • Changsheng Guo

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • ,
  • Susan Orloff

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • ,
  • Laura Eaton

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • ,
  • Eric Benedetti

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • ,
  • Bryan Jensen

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • ,
  • Milton Finegold

      Affiliations

    • Department of Pathology, Texas Children's Hospital, Houston, Texas
    • ,
  • Holger Willenbring

      Affiliations

    • Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California
    • Department of Surgery, Division of Transplantation, University of California, San Francisco, California
    • ,
  • Markus Grompe

      Affiliations

    • Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon
    • Papé Family Pediatric Research Institute, Portland, Oregon

Received 24 December 2009 ,Accepted 27 May 2010.

References 

  1. Fisher RA, Strom SC. Human hepatocyte transplantation: worldwide results. Transplantation. 2006;82:441–449
  2. Basma H, Soto-Gutierrez A, Yannam GR, et al. Differentiation and transplantation of human embryonic stem cell-derived hepatocytes. Gastroenterology. 2009;136:990–999
  3. Hay DC, Fletcher J, Payne C, et al. Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling. Proc Natl Acad Sci U S A. 2008;105:12301–12306
  4. Cantz T, Zuckerman DM, Burda MR, et al. Quantitative gene expression analysis reveals transition of fetal liver progenitor cells to mature hepatocytes after transplantation in uPA/RAG-2 mice. Am J Pathol. 2003;162:37–45
  5. Sharma AD, Cantz T, Vogel A, et al. Murine embryonic stem cell-derived hepatic progenitor cells engraft in recipient livers with limited capacity of liver tissue formation. Cell Transplant. 2008;17:313–323
  6. Hentze H, Graichen R, Colman A. Cell therapy and the safety of embryonic stem cell-derived grafts. Trends Biotechnol. 2007;25:24–32
  7. Yesus YW, Kenneally C, Taylor HM. Preservation of hematopoietic stem cells in cadaveric marrow. Am J Clin Pathol. 1981;76:205–207
  8. Mugishima H, Terasaki P, Sueyoshi A. Bone marrow from cadaver donors for transplantation. Blood. 1985;65:392–396
  9. Liu PI, Ogawa M, Crook L, et al. Proliferative function of cadaveric bone marrow cells. Am J Hematol. 1978;5:145–150
  10. Hughes RD, Mitry RR, Dhawan A, et al. Isolation of hepatocytes from livers from non-heart-beating donors for cell transplantation. Liver Transpl. 2006;12:713–717
  11. Porretti L, Gatti S, Gramignoli R, et al. Animal model for liver cell banking from non-heart beating donors after prolonged ischaemia time. Dig Liver Dis. 2006;38:905–911
  12. Zambrowicz BP, Imamoto A, Fiering S, et al. Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Proc Natl Acad Sci U S A. 1997;94:3789–3794
  13. Grompe M, al-Dhalimy M, Finegold M, et al. Loss of fumarylacetoacetate hydrolase is responsible for the neonatal hepatic dysfunction phenotype of lethal albino mice. Genes Dev. 1993;7:2298–2307
  14. Seglen PO. Preparation of isolated rat liver cells. Methods Cell Biol. 1976;13:29–83
  15. Overturf K, Al-Dhalimy M, Tanguay R, et al. Hepatocytes corrected by gene therapy are selected in vivo in a murine model of hereditary tyrosinaemia type I. Nat Genet. 1996;12:266–273
  16. Grompe M, Lindstedt S, al-Dhalimy M, et al. Pharmacological correction of neonatal lethal hepatic dysfunction in a murine model of hereditary tyrosinaemia type I. Nat Genet. 1995;10:453–460
  17. MacGregor GR, Mogg AE, Burke JF, et al. Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of the bacterial gene. Somat Cell Mol Genet. 1987;13:253–265
  18. Wang X, Foster M, Al-Dhalimy M, et al. The origin and liver repopulating capacity of murine oval cells. Proc Natl Acad Sci U S A. 2003;100(Suppl 1):11881–11888
  19. Scallan CD, Lillicrap D, Jiang H, et al. Sustained phenotypic correction of canine hemophilia A using an adeno-associated viral vector. Blood. 2003;102:2031–2037
  20. Lewandoski M, Meyers EN, Martin GR. Analysis of Fgf8 gene function in vertebrate development. Cold Spring Harb Symp Quant Biol. 1997;62:159–168
  21. Grimm D, Streetz KL, Jopling CL, et al. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature. 2006;441:537–541
  22. Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet. 1999;21:70–71
  23. Strom S, Dorko K, Thompson M. Large scale isolation and culture of human hepatocytes. In:  Franco D,  Boudjema K,  Varet B editor. Ilots de Langerhans et hepatocytes. 1998;p. 195–205
  24. Hill RP, Marie-Egyptienne DT, Hedley DW. Cancer stem cells, hypoxia and metastasis. Semin Radiat Oncol. 2009;19:106–111
  25. Perera MT, Mirza DF, Elias E. Liver transplantation: issues for the next 20 years. J Gastroenterol Hepatol. 2009;24(Suppl 3):S124–S131
  26. Muiesan P, Girlanda R, Jassem W, et al. Single-center experience with liver transplantation from controlled non-heart beating donors: a viable source of grafts. Ann Surg. 2005;242:732–738
  27. Stachelscheid H, Urbaniak T, Ring A, et al. Isolation and characterization of adult human liver progenitors from ischemic liver tissue derived from therapeutic hepatectomies. Tissue Eng Part A. 2009;15:1633–1643

 Conflicts of interest The authors disclose no conflicts.

 Funding This project was supported by NIH grant R01 DK051592 (L.E., H.A., and M.G.). NIH also partially funded this work through grant RR00163 for the Oregon National Primate Research Center. Also supported by grants from the California Institute for Regenerative Medicine, American Liver Foundation, and American Society of Transplantation (H.W.). M.F. was funded by the NIDDK Core Lab DK 56338 of the Texas Gulf Coast Digestive Diseases Center.

PII: S0016-5085(10)00844-9

doi: 10.1053/j.gastro.2010.05.082

Gastroenterology
Volume 139, Issue 3 , Pages 1019-1029 , September 2010

Article Tools

* Image Usage & Resolution