Paired Immunoglobulin-Like Receptor B (PIR-B) Negatively Regulates Macrophage Activation in Experimental Colitis

Background & Aims

Innate and adaptive immune responses are regulated by cross talk between activation and inhibitory signals. Dysregulation of the inhibitory signal can lead to aberrant chronic inflammatory diseases such as the inflammatory bowel diseases (IBD). Little is known about negative regulation of innate intestinal immune activation. We examined the role of the inhibitory receptor paired immunoglobulin-like receptor B (PIR-B) in the regulation of macrophage function in innate intestinal immunity.

Methods

We examined the susceptibility of Pirb^−/− and wild-type (WT) mice to dextran sodium sulfate (DSS)-induced colitis. We assessed proinflammatory cytokine release and mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) activation in Pirb^−/− and WT macrophages following Escherichia coli stimulation. Macrophage transfer experiments were performed to define the role of PIR-B in the negative regulation of macrophage function in DSS-induced colitis. We also assessed expression of PIR-B human homologues (immunoglobulin-like transcript [ILT]-2 and ILT-3) in colon biopsy samples from healthy individuals (controls) and patients with IBD.

Results

Pirb^−/− mice had increased susceptibility to DSS-induced colitis. In vitro analysis showed increased production of proinflammatory cytokines (interleukin-6, interleukin-1β, and tumor necrosis factor α) and activation of MAPK and NF-κB in Pirb^−/− macrophages following bacterial activation. Adoptive transfer of bone marrow–derived Pirb^−/− macrophages into WT mice was sufficient to increase disease susceptibility. ILT-2 and ILT-3 were expressed on CD68⁺ and CD68⁻ mononuclear cells and intestinal epithelium in colon biopsy samples from patients and controls.

Conclusions

PIR-B negatively regulates macrophage functions in response to pathogenic bacteria and chronic intestinal inflammatory responses. Inhibitory receptors such as PIR-B might be used as therapeutic targets for treatment of patients with IBD.

Keywords: Inhibitory Receptors, Macrophages, IBD

Abbreviations used in this paper: DSS, dextran sodium sulfate, eGFP, enhanced green fluorescent protein, ERK, extracellular signal–regulated kinase, Ig, immunoglobulin, IL, interleukin, ILT, immunoglobulin-like transcript, ITIM, immunoreceptor tyrosine-based inhibitory motif, JNK, c-Jun-N-terminal kinase, LIR, leukocyte immunoglobulin-like receptor, LP, lamina propria, LPS, lipopolysaccharide, MAPK, mitogen-activated protein kinase, NF-κB, nuclear factor κB, PIR, paired immunoglobulin-like receptor, SHP, Src-homology 2 (SH2) domain-containing tyrosine phosphatase, TLR, Toll-like receptor, TNF, tumor necrosis factor, WT, wild type