Gastroenterology
Volume 138, Issue 2 , Pages 435-446 , February 2010

In Vivo Molecular Imaging of Colorectal Cancer With Confocal Endomicroscopy by Targeting Epidermal Growth Factor Receptor

  • Martin Goetz

      Affiliations

    • I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Mainz, Germany
    • Corresponding Author InformationReprint requests Address requests for reprints to: Martin Goetz, MD, PhD, I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Langenbeckstr. 1, 55131 Mainz, Germany. fax: (49) 6131-17-5552
  • ,
  • Alex Ziebart

      Affiliations

    • I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Mainz, Germany
  • ,
  • Sebastian Foersch

      Affiliations

    • I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Mainz, Germany
  • ,
  • Michael Vieth

      Affiliations

    • Institute of Pathology, Klinikum Bayreuth, Bayreuth, Germany
  • ,
  • Maximilian J. Waldner

      Affiliations

    • Institute of Molecular Medicine, Universitätsmedizin Mainz, Mainz, Germany
  • ,
  • Peter Delaney

      Affiliations

    • Optiscan Pty, Ltd, Notting Hill, Victoria, Australia
  • ,
  • Peter R. Galle

      Affiliations

    • I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Mainz, Germany
  • ,
  • Markus F. Neurath

      Affiliations

    • I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Mainz, Germany
    • Medizinische Klinik 1, Universitätsklinikum Erlangen, Erlangen, Germany
  • ,
  • Ralf Kiesslich

      Affiliations

    • I. Medizinische Klinik und Poliklinik, Universitätsmedizin Mainz, Mainz, Germany

Received 6 June 2009 ,Accepted 14 October 2009.

References 

  1. Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2008. CA Cancer J Clin. 2008;58:71–96
  2. Selby JV, Friedman GD, Quesenberry CP, et al. A case-control study of screening sigmoidoscopy and mortality from colorectal cancer. N Engl J Med. 1992;326:653–657
  3. Levin B, Lieberman DA, McFarland B, et al. Screening and surveillance for the early detection of colorectal cancer and adenomatous polyps, 2008: a joint guideline from the American Cancer Society, the US Multi-Society Task Force on Colorectal Cancer, and the American College of Radiology. Gastroenterology. 2008;134:1570–1595
  4. Herbst RS, Shin DM. Monoclonal antibodies to target epidermal growth factor receptor-positive tumors: a new paradigm for cancer therapy. Cancer. 2002;94:1593–1611
  5. Ciardiello F, Tortora G. EGFR antagonists in cancer treatment. N Engl J Med. 2008;358:1160–1174
  6. Salomon DS, Brandt R, Ciardiello F, et al. Epidermal growth factor-related peptides and their receptors in human malignancies. Crit Rev Oncol Hematol. 1995;19:183–232
  7. Normanno N, De Luca A, Bianco C, et al. Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 2006;366:2–16
  8. Nicholson RI, Gee JM, Harper ME. EGFR and cancer prognosis. Eur J Cancer. 2001;37(Suppl 4):S9–S15
  9. Abd El All HS, Mishriky AM, Mohamed FA. Epidermal growth factor receptor in colorectal carcinoma: correlation with clinico-pathological prognostic factors. Colorectal Dis. 2008;10:170–178
  10. Goldstein NS, Armin M. Epidermal growth factor receptor immunohistochemical reactivity in patients with American Joint Committee on Cancer Stage IV colon adenocarcinoma: implications for a standardized scoring system. Cancer. 2001;92:1331–1346
  11. Grunwald V, Hidalgo M. Developing inhibitors of the epidermal growth factor receptor for cancer treatment. J Natl Cancer Inst. 2003;95:851–867
  12. Galizia G, Lieto E, Orditura M, et al. Epidermal growth factor receptor (EGFR) expression is associated with a worse prognosis in gastric cancer patients undergoing curative surgery. World J Surg. 2007;31:1458–1468
  13. Saltz LB, Meropol NJ, Loehrer PJ, et al. Phase II trial of cetuximab in patients with refractory colorectal cancer that expresses the epidermal growth factor receptor. J Clin Oncol. 2004;22:1201–1208
  14. Cunningham D, Humblet Y, Siena S, et al. Cetuximab monotherapy and cetuximab plus irinotecan in irinotecan-refractory metastatic colorectal cancer. N Engl J Med. 2004;351:337–345
  15. Jonker DJ, O'Callaghan CJ, Karapetis CS, et al. Cetuximab for the treatment of colorectal cancer. N Engl J Med. 2007;357:2040–2048
  16. Lievre A, Bachet JB, Boige V, et al. KRAS mutations as an independent prognostic factor in patients with advanced colorectal cancer treated with cetuximab. J Clin Oncol. 2008;26:374–379
  17. De Roock W, Piessevaux H, De Schutter J, et al. KRAS wild-type state predicts survival and is associated to early radiological response in metastatic colorectal cancer treated with cetuximab. Ann Oncol. 2008;19:508–515
  18. Italiano A, Follana P, Caroli FX, et al. Cetuximab shows activity in colorectal cancer patients with tumors for which FISH analysis does not detect an increase in EGFR gene copy number. Ann Surg Oncol. 2008;15:649–654
  19. Chung KY, Shia J, Kemeny NE, et al. Cetuximab shows activity in colorectal cancer patients with tumors that do not express the epidermal growth factor receptor by immunohistochemistry. J Clin Oncol. 2005;23:1803–1810
  20. Schrag D. The price tag on progress—chemotherapy for colorectal cancer. N Engl J Med. 2004;351:317–319
  21. Yoshida S, Tanaka S, Hirata M, et al. Optical biopsy of GI lesions by reflectance-type laser-scanning confocal microscopy. Gastrointest Endosc. 2007;66:144–149
  22. Nakao M, Yoshida S, Tanaka S, et al. Optical biopsy of early gastroesophageal cancer by catheter-based reflectance-type laser-scanning confocal microscopy. J Biomed Opt. 2008;13:054043
  23. Curvers WL, Singh R, Song LM, et al. Endoscopic tri-modal imaging for detection of early neoplasia in Barrett's oesophagus: a multi-centre feasibility study using high-resolution endoscopy, autofluorescence imaging and narrow band imaging incorporated in one endoscopy system. Gut. 2008;57:167–172
  24. van den Broek FJ, Fockens P, van Eeden S, et al. Endoscopic tri-modal imaging for surveillance in ulcerative colitis: randomised comparison of high-resolution endoscopy and autofluorescence imaging for neoplasia detection; and evaluation of narrow-band imaging for classification of lesions. Gut. 2008;57:1083–1089
  25. Matsuda T, Saito Y, Fu KI, et al. Does autofluorescence imaging videoendoscopy system improve the colonoscopic polyp detection rate? (A pilot study). Am J Gastroenterol. 2008;103:1926–1932
  26. Mayinger B, Neumann F, Kastner C, et al. Early detection of premalignant conditions in the colon by fluorescence endoscopy using local sensitization with hexaminolevulinate. Endoscopy. 2008;40:106–109
  27. Mayinger B, Neidhardt S, Reh H, et al. Fluorescence induced with 5-aminolevulinic acid for the endoscopic detection and follow-up of esophageal lesions. Gastrointest Endosc. 2001;54:572–578
  28. Kiesslich R, Burg J, Vieth M, et al. Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo. Gastroenterology. 2004;127:706–713
  29. Kiesslich R, Gossner L, Goetz M, et al. In vivo histology of Barrett's esophagus and associated neoplasia by confocal laser endomicroscopy. Clin Gastroenterol Hepatol. 2006;4:979–987
  30. Polglase AL, McLaren WJ, Skinner SA, et al. A fluorescence confocal endomicroscope for in vivo microscopy of the upper- and the lower-GI tract. Gastrointest Endosc. 2005;62:686–695
  31. Hsiung PL, Hardy J, Friedland S, et al. Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy. Nat Med. 2008;14:454–458
  32. Leibovitz A, Stinson JC, McCombs WB, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 1976;36:4562–4569
  33. Price JE, Daniels LM, Campbell DE, et al. Organ distribution of experimental metastases of a human colorectal carcinoma injected in nude mice. Clin Exp Metastasis. 1989;7:55–68
  34. Flatmark K, Maelandsmo GM, Martinsen M, et al. Twelve colorectal cancer cell lines exhibit highly variable growth and metastatic capacities in an orthotopic model in nude mice. Eur J Cancer. 2004;40:1593–1598
  35. Goetz M, Fottner C, Schirrmacher E, et al. In vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases. Endoscopy. 2007;39:350–356
  36. Goetz M, Memadathil B, Biesterfeld S, et al. In vivo subsurface morphological and functional cellular and subcellular imaging of the gastrointestinal tract with confocal mini-microscopy. World J Gastroenterol. 2007;13:2160–2165
  37. Kiesslich R, Goetz M, Angus EM, et al. Identification of epithelial gaps in human small and large intestine by confocal endomicroscopy. Gastroenterology. 2007;133:1769–1778
  38. Endlicher E, Gelbmann CM, Knuchel R, et al. Hexaminolevulinate-induced fluorescence endoscopy in patients with rectal adenoma and cancer: a pilot study. Gastrointest Endosc. 2004;60:449–454
  39. Bibeau F, Boissiere-Michot F, Sabourin JC, et al. Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray. Virchows Arch. 2006;449:281–287
  40. Erlichman C, Sargent DJ. New treatment options for colorectal cancer. N Engl J Med. 2004;351:391–392
  41. Valentini AM, Pirrelli M, Caruso ML. EGFR-targeted therapy in colorectal cancer: does immunohistochemistry deserve a role in predicting the response to cetuximab?. Curr Opin Mol Ther. 2008;10:124–131
  42. Saltz L. Epidermal growth factor receptor-negative colorectal cancer: is there truly such an entity?. Clin Colorectal Cancer. 2005;5(Suppl 2):S98–S100
  43. Atkins D, Reiffen KA, Tegtmeier CL, et al. Immunohistochemical detection of EGFR in paraffin-embedded tumor tissues: variation in staining intensity due to choice of fixative and storage time of tissue sections. J Histochem Cytochem. 2004;52:893–901
  44. Derecskei K, Moldvay J, Bogos K, et al. Protocol modifications influence the result of EGF receptor immunodetection by EGFR pharmDx in paraffin-embedded cancer tissues. Pathol Oncol Res. 2006;12:243–246
  45. Bhargava R, Chen B, Klimstra DS, et al. Comparison of two antibodies for immunohistochemical evaluation of epidermal growth factor receptor expression in colorectal carcinomas, adenomas, and normal mucosa. Cancer. 2006;106:1857–1862
  46. Penault-Llorca F, Cayre A, Arnould L, et al. Is there an immunohistochemical technique definitively valid in epidermal growth factor receptor assessment?. Oncol Rep. 2006;16:1173–1179
  47. Moroni M, Sartore-Bianchi A, Veronese S, et al. EGFR FISH in colorectal cancer: what is the current reality?. Lancet Oncol. 2008;9:402–403
  48. Hsu ER, Anslyn EV, Dharmawardhane S, et al. A far-red fluorescent contrast agent to image epidermal growth factor receptor expression. Photochem Photobiol. 2004;79:272–279
  49. Yu YA, Shabahang S, Timiryasova TM, et al. Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins. Nat Biotechnol. 2004;22:313–320
  50. Ke S, Wen X, Gurfinkel M, et al. Near-infrared optical imaging of epidermal growth factor receptor in breast cancer xenografts. Cancer Res. 2003;63:7870–7875
  51. Keller R, Winde G, Terpe HJ, et al. Fluorescence endoscopy using a fluorescein-labeled monoclonal antibody against carcinoembryonic antigen in patients with colorectal carcinoma and adenoma. Endoscopy. 2002;34:801–807
  52. Barrett T, Koyama Y, Hama Y, et al. In vivo diagnosis of epidermal growth factor receptor expression using molecular imaging with a cocktail of optically labeled monoclonal antibodies. Clin Cancer Res. 2007;13:6639–6648
  53. Kiesslich R, Hurlstone DP, Dunbar K, et al. Confocal Laser Endomicroscopy is an effective and safe tool in GI-Endoscopy. Gastroenterology. 2008;134:A191
  54. Pacurariu RI. Low incidence of side effects following intravenous fluorescein angiography. Ann Ophthalmol. 1982;14:32–36

 Conflicts of interest The authors disclose the following: Peter Delaney is director of technology at Optiscan Pty, Ltd, Notting Hill, Victoria, Australia. Ralf Kiesslich and Markus F. Neurath report having received research grants from Pentax, Tokio, Japan. The remaining authors disclose no conflicts.

 Funding Supported by a government grant of the “Stiftung Rheinland-Pfalz für Innovation” (961-38 62 61/821-Projekt 821) (to M.G.).

PII: S0016-5085(09)01858-7

doi: 10.1053/j.gastro.2009.10.032

Gastroenterology
Volume 138, Issue 2 , Pages 435-446 , February 2010