Gastroenterology
Volume 135, Issue 5 , Pages 1543-1551 , November 2008

A Novel Noninvasive Genotyping Method of Helicobacter pylori Using Stool Specimens

  • Sonja Puz

      Affiliations

    • Division of Clinical Microbiology, Department of Hygiene and Medical Microbiology, Medical University of Vienna, Vienna, Austria
    • ,
  • Albina Innerhofer

      Affiliations

    • St. Anna Children Hospital, Medical University Vienna, Vienna, Austria
    • ,
  • Michael Ramharter

      Affiliations

    • Division of Infectious Diseases and Tropical Medicine, Department of Medicine I, Medical University Vienna, Vienna, Austria
    • Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon
    • ,
  • Michael Haefner

      Affiliations

    • Division of Gastroenterology and Hepatology, Department of Medicine IV, Medical University Vienna, Vienna, Austria
    • ,
  • Alexander M. Hirschl

      Affiliations

    • Division of Clinical Microbiology, Department of Hygiene and Medical Microbiology, Medical University of Vienna, Vienna, Austria
    • ,
  • Zsuzsanna Kovách

      Affiliations

    • Division of Clinical Microbiology, Department of Hygiene and Medical Microbiology, Medical University of Vienna, Vienna, Austria
    • ,
  • Manfred Rotter

      Affiliations

    • Division of Clinical Microbiology, Department of Hygiene and Medical Microbiology, Medical University of Vienna, Vienna, Austria
    • ,
  • Athanasios Makristathis

      Affiliations

    • Division of Clinical Microbiology, Department of Hygiene and Medical Microbiology, Medical University of Vienna, Vienna, Austria
    • Corresponding Author InformationAddress reprint requests to: Athanasios Makristathis, PhD, Division of Clinical Microbiology, Department of Hygiene and Medical Microbiology, Medical University Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria

Received 4 February 2008 ,Accepted 7 August 2008.

References 

  1. Rothenbacher D, Inceoglu J, Bode G, et al. Acquisition of Helicobacter pylori infection in a high-risk population occurs within the first 2 years of life. J Pediatr. 2000;136:744–748
  2. Thomas JE, Dale A, Harding M, et al. Helicobacter pylori colonization in early life. Pediatr Res. 1999;45:218–223
  3. Granström M, Tindberg Y, Blennow M. Seroepidemiology of Helicobacter pylori infection in a cohort of children monitored from 6 months to 11 years of age. J Clin Microbiol. 1997;35:468–470
  4. Tindberg Y, Nyren O, Blennow M, et al. Helicobacter infection in Swedish school children: lack of evidence of child-to-child transmission outside the family. Gastroenterology. 2001;12:310–316
  5. Farrell S, Doherty GM, Milliken I, et al. Risk factors for Helicobacter pylori infection in children—an examination of the role played by intrafamilial bed sharing. Pediatr Infect Dis J. 2005;24:149–152
  6. Weyermann M, Adler G, Brenner H, et al. The mother as source of Helicobacter pylori infection. Epidemiology. 2006;17:332–334
  7. Rothenbacher D, Winkler M, Gonser T, et al. Role of infected parents in transmission of Helicobacter pylori to their children. Pediatr Infect Dis J. 2002;21:674–679
  8. Akopyanz N, Bukanov NO, Westblom U, et al. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res. 1992;20:5137–5142
  9. Konno M, Fujii N, Yokota S, et al. Five-year follow-up study of mother-to-child transmission of Helicobacter pylori infection detected by a random amplified polymorphic DNA fingerprinting method. J Clin Microbiol. 2005;43:2246–2250
  10. Bamford KB, Bickley J, Collins JS, et al. Helicobacter pylori: comparison of DNA fingerprints provides evidence for intrafamilial infection. Gut. 1993;34:1348–1350
  11. Chalkauskas H, Kersulyte D, Cepuliene I, et al. Genotypes of Helicobacter pylori in Lithuanian families. Helicobacter. 1998;3:296–302
  12. Gibson JR, Slater E, Xerry J, et al. Use of an amplified-fragment length polymorphism technique to fingerprint and differentiate isolates of Helicobacter pylori. J Clin Microbiol. 1998;36:2580–2585
  13. Han SR, Zschausch HC, Meyer HG, et al. Helicobacter pylori: clonal population structure and restricted transmission within families revealed by molecular typing. J Clin Microbiol. 2000;38:3646–3651
  14. Kivi M, Tindberg Y, Sorberg M, et al. Concordance of Helicobacter pylori strains between families. J Clin Microbiol. 2003;41:5604–5608
  15. Roma-Giannikou E, Karameris A, Balatsos B, et al. Intrafamilial spread of Helicobacter pylori: a genetic analysis. Helicobacter. 2003;8:15–20
  16. Schabereiter-Gurtner C, Hirschl AM, Dragosics B, et al. Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J Clin Microbiol. 2004;42:4512–4518
  17. De Reuse H, Labigne A, Mengin-Lecreulx D. The Helicobacter pylori ureC gene codes for a phosphoglucosamine mutase. J Bacteriol. 1997;179:3488–3493
  18. Fischer W, Haas R. The recA protein of Helicobacter pylori requires a posttranslational modification for full activity. J Bacteriol. 2004;186:777–784
  19. Burucoa C, Lhomme V, Fauchere JL. Performance criteria of DNA fingerprinting methods for typing of Helicobacter pylori isolates: experimental results and meta-analysis. J Clin Microbiol. 1999;37:4071–4080
  20. Edwards KJ, Metherell LA, Yates M, et al. Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis. J Clin Microbiol. 2001;39:3350–3352
  21. Logan JM, Edwards KJ, Saunders NA, et al. Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-time PCR. J Clin Microbiol. 2001;39:2227–2232
  22. Gibson JR, Saunders NA, Burke B, et al. Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori. J Clin Microbiol. 1999;37:3746–3748
  23. Chisholm SA, Owen RJ, Teare EL, et al. PCR-based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples. J Clin Microbiol. 2001;39:1217–1220
  24. Van der Ende A, Pan ZJ, Bart A, et al. cagA-positive Helicobacter pylori populations in China and The Netherlands are distinct. Infect Immun. 1998;66:1822–1826
  25. Maggi Solca N, Bernasconi MV, Valsangiacomo C, et al. Population genetics of Helicobacter pylori in the southern part of Switzerland analysed by sequencing of four housekeeping genes (atpD, glnA, scoB and recA), and by vacA, cagA, iceA and IS605 genotyping. Microbiology. 2001;147:1693–1707
  26. Schmitt W, Odenbreit S, Heuermann D, et al. Cloning of the Helicobacter pylori recA gene and functional characterization of its product. Mol Gen Genet. 1995;248:563–572
  27. Rothenbacher D, Brenner H. Burden of Helicobacter pylori and H. pylori-related diseases in developed countries: recent developments and future implications. Microbes Infect. 2003;5:693–703
  28. Raymond J, Thiberg JM, Chevalier C, et al. Genetic and transmission analysis of Helicobacter pylori strains within a family. Emerg Infect Dis. 2004;10:1816–1821
  29. Wong BC, Wang WH, Berg DE, et al. High prevalence of mixed infections by Helicobacter pylori in Hong Kong: metronidazole sensitivity and overall genotype. Aliment Pharmacol Ther. 2001;15:493–503
  30. Carroll IM, Ahmed N, Beesley SM, et al. Microevolution between paired antral and paired antrum and corpus Helicobacter pylori isolates recovered from individual patients. J Med Microbiol. 2004;53:669–677

 The authors disclose the following: Supported by a research grant from the Jubiläumsfonds of the Austrian National Bank. Field work at the Medical Research Unit, Lambaréné, Gabon was supported by a grant from the Hochschulstiftung der Stadt Wien.

PII: S0016-5085(08)01513-8

doi: 10.1053/j.gastro.2008.08.006

Gastroenterology
Volume 135, Issue 5 , Pages 1543-1551 , November 2008

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