Gastroenterology
Volume 134, Issue 1 , Pages 166-178 , January 2008

PepT1-Mediated Tripeptide KPV Uptake Reduces Intestinal Inflammation

  • Guillaume Dalmasso
    • ,
  • Laetitia Charrier–Hisamuddin

      Affiliations

    • Laetitia Charrier-Hisamuddin is a recipient of a research career development award from the Crohn’s and Colitis Foundation of America and Yutao Yan is a recipient of a research fellowship award from the Crohn’s and Colitis Foundation of America.
    • ,
  • Hang Thi Thu Nguyen
    • ,
  • Yutao Yan
    • ,
  • Shanthi Sitaraman
    • ,
  • Didier Merlin

      Affiliations

    • Corresponding Author InformationAddress requests for reprints to: Didier Merlin, PhD, Department of Medicine, Division of Digestive Diseases, Emory University, 615 Michael Street, Atlanta, Georgia 30322. fax: (404) 727-5767.

Received 18 May 2007 ,Accepted 4 October 2007.

References 

  1. Liang R, Fei YJ, Prasad PD, et al. Human intestinal H+/peptide cotransporter (Cloning, functional expression, and chromosomal localization). J Biol Chem. 1995;270:6456–6463
  2. Steel A, Nussberger S, Romero MF, et al. Stoichiometry and pH dependence of the rabbit proton-dependent oligopeptide transporter PepT1. J Physiol. 1997;498:563–569
  3. Abidi SA. The oligopeptide (PepT1) in human intestine: biology and function. Gastroenterology. 1997;113:332–340
  4. Abidi SA. Regulation of expression of the intestinal oligopeptide transporter (PepT1) in health and disease. Am J Physiol. 2003;285:G779–G788
  5. Daniel H. Molecular and integrative physiology of intestinal peptide transport. Annu Rev Physiol. 2004;66:361–384
  6. Daniel H, Kottra G. The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology. Pflugers Arch. 2004;447:610–618
  7. Fei YJ, Ganapathy V, Leibach FH. Molecular and structural features of the proton coupled oligopeptide transporter superfamily. Prog Nucleic Acid Res Mol Biol. 1998;58:239–261
  8. Meredith D, Boyd CAR. Structure and function of eukaryotic peptide transporter. Cell Mol Life Sci. 2000;57:754–778
  9. Knutter I, Rubio-Aliaga I, Boll M, et al. H+ peptide cotransport in the human bile duct epithelium cell line SK-ChA-1. Am J Physiol. 2002;283:G222–G229
  10. Ogihara H, Saito H, Shin BC, et al. Immuno-localization of H+/peptide cotransporter in rat digestive tract. Biochem Biophy Res Commun. 1996;220:848–852
  11. Saito H, Okuda M, Terada T, et al. Cloning and characterization of a rat H+/peptide cotransporter mediating absorption of β-lactam antibiotics in the intestine and kidney. J Pharmacol Exp Ther. 1995;275:1631–1637
  12. Chen H, Smith DE, Yang T, et al. Localization of PEPT1 and PEPT2 proton-coupled oligopeptide transporter mRNA and protein in rat kidney. Am J Physiol. 1999;276:F658–F665
  13. Smith DE, Pavlova A, Berger UV, et al. Tubular localization and tissue distribution of peptide transporter in rat kidney. Pharmaceut Res. 1998;15:1244–1249
  14. Freeman TC, Bentsen BS, Thwaites DT, et al. H+/di-tripeptide transporter (PepT1) expression in the rabbit intestine. Pflugers Arch. 1995;430:394–400
  15. Sai Y, Tamai I, Hayashi K, et al. Immunolocalization and pharmacological relevance of oligopeptide transporter PepT1 in intestinal absorption of beta-lactam antibiotics. FEBS Lett. 1996;392:25–29
  16. Ziegler TR, Fernandez-Estivariz C, Gu LH, et al. Distribution of the H+/peptide transporter PepT1 in human intestine: up-regulated expression in the colonic mucosa of patients with short-bowel syndrome. Am J Clin Nutr. 2002;75:922–930
  17. Merlin D, Si-Tahar M, Sitaraman SV, et al. Colonic epithelial hPepT1 expression occurs in inflammatory bowel disease: transport of bacterial peptides influence expression of MHC class 1 molecules. Gastroenterology. 2001;120:1666–1679
  18. Charrier L, Driss A, Yan Y, et al. hPepT1 mediates bacterial tripeptide fMLP uptake in human monocytes. Lab Invest. 2006;86:490–503
  19. Hiltz ME, Lipton JM. Antiinflammatory activity of a COOH-terminal fragment of the neuropeptide alpha-MSH. FASEB J. 1989;11:2282–2284
  20. Mandrika I, Muceniece R, Wikberg JES. Effects of melanocortin peptides on lipopolysaccharide/interferon-gamma-induced NF-kappaB DNA binding and nitric oxide production in macrophage-like RAW 264.7 cells: evidence for dual mechanisms of action. Biochem Pharmacol. 2001;61:613–621
  21. Kelly JM, Moir AJ, Carlson K, et al. Immobilized α-melanocyte stimulating hormone 10-13 (GKPV) inhibits tumor necrosis factor-α stimulated NF-κB activity. Peptide. 2006;27:431–437
  22. Cooper A, Robinson SJ, Pickard C, et al. α-melanocyte-stimulating hormone suppresses antigen-induced lymphocyte proliferation in humans independently of melanocortin 1 receptor gene status. J Immunol. 2005;175:4806–4813
  23. Jobin C, Haskill S, Mayer L, et al. Evidence for altered regulation of IκBα degradation in human colonic epithelial cells. J Immunol. 1997;158:226–234
  24. Hoffmann E, Dittrich-Breiholz O, Holtmann H, et al. Multiple control of interleukin-8 gene expression. J Leukoc Biol. 2002;72:847–855
  25. McCormick BA, Colgan SP, Delp-Archer C, et al. Salmonella typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils. J Cell Biol. 1993;123:895–907
  26. Haddad JJE, Lauterbach R, Saadé NE, et al. α-melanocyte-related tripeptide, Lys-D-Pro-Val, ameliorates endotoxin-induced nuclear factor κB translocation and activation: evidence for involvement of an interleukin-1β193-195 receptor antagonism in the alveolar epithelium. Biochem J. 2001;355:29–38
  27. Strober W, Fuss IJ, Blumberg RS. The immunology of mucosal models of inflammation. Annu Rev Immunol. 2002;20:495–549
  28. Rajora N, Boccoli G, Catania A, et al. α-MSH modulates experimental inflammatory bowel disease. Peptides. 1997;18:381–385
  29. Oktar BK, Ercan F, Yeğen BC, et al. The effect of α-melanocyte stimulating hormone on colonic inflammation in the rat. Peptides. 2000;21:1271–1277
  30. Getting SJ. Targeting melanocortin receptors as potential novel therapeutics. Pharmacol Ther. 2006;111:1–15
  31. Getting SJ, Schiöth HB, Perretti M. Dissection of the anti-inflammatory effect of the core and C-terminal (KPV) α-melanocyte-stimulating hormone peptides. J Pharmacol Exp Ther. 2003;306:631–637
  32. Schiöth HB, Muceniece R, Mutule I, et al. New melanocortin 1 receptor binding motif based on the C-terminal sequence of α-melanocyte-stimulating hormone. Basic Clin Toxicol Pharmacol. 2006;99:287–293
  33. Brandsch M, Miyamoto Y, Ganapathy V, et al. Expression and protein kinase C-dependent regulation of peptide /H+ cotransport system in the Caco-2 human colon carcinoma cell line. Biochem J. 1994;299:253–260

 Supported by the National Institutes of Health of Diabetes and Digestive and Kidney Diseases by grants R24-DK-064399 (center grant), RO1-DK-061941, RO1-DK-071594 (D.M.), and RO1-DK55850 (S.S.).

PII: S0016-5085(07)01852-5

doi: 10.1053/j.gastro.2007.10.026

Gastroenterology
Volume 134, Issue 1 , Pages 166-178 , January 2008

Article Tools

* Image Usage & Resolution