Gastroenterology
Volume 130, Issue 7 , Pages 2010-2022 , June 2006

Cleavage of Endoplasmic Reticulum Proteins in Hepatocellular Carcinoma: Detection of Generated Fragments in Patient Sera

  • Nicolas Chignard

      Affiliations

    • Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan
    • N.C. and S.S. contributed equally to this paper.
    • ,
  • Sufen Shang

      Affiliations

    • Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington
    • N.C. and S.S. contributed equally to this paper.
    • ,
  • Hong Wang

      Affiliations

    • Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington
    • ,
  • Jorge Marrero

      Affiliations

    • Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan
    • ,
  • Christian Bréchot

      Affiliations

    • INSERM U.370, Necker Institute, Paris, France
    • ,
  • Samir Hanash

      Affiliations

    • Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington
    • ,
  • Laura Beretta

      Affiliations

    • Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan
    • Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington
    • Corresponding Author InformationAddress requests for reprints to: Laura Beretta, PhD, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North - M5-A864, Seattle, Washington 98109-1024. fax: (206) 667-2537

Received 25 April 2005 ,Accepted 15 February 2006.

References 

  1. Bosch FX, Ribes J, Diaz M, Cleries R. Primary liver cancer (worldwide incidence and trends). Gastroenterology. 2004;127:S5–S16
  2. Llovet JM, Burroughs A, Bruix J. Hepatocellular carcinoma. Lancet. 2003;362:1907–1917
  3. El-Serag HB, Mason AC, Key C. Trends in survival of patients with hepatocellular carcinoma between 1977 and 1996 in the United States. Hepatology. 2001;33:62–65
  4. El-Serag HB. Hepatocellular carcinoma (recent trends in the United States). Gastroenterology. 2004;127:S27–S34
  5. Bruix J, Sherman M, Llovet JM, Beaugrand M, Lencioni R, Burroughs AK, et al. Clinical management of hepatocellular carcinoma. Conclusions of the Barcelona-2000 EASL conference. European Association for the Study of the Liver. J Hepatol. 2001;35:421–430
  6. Di Bisceglie AM. Issues in screening and surveillance for hepatocellular carcinoma. Gastroenterology. 2004;127:S104–S107
  7. Daniele B, Bencivenga A, Megna AS, Tinessa V. Alpha-fetoprotein and ultrasonography screening for hepatocellular carcinoma. Gastroenterology. 2004;127:S108–S112
  8. Hong WS, Hong SI. Clinical usefulness of α-1-antitrypsin in the diagnosis of hepatocellular carcinoma. J Korean Med Sci. 1991;6:206–213
  9. Marrero JA, Su GL, Wei W, Emick D, Conjeevaram HS, Fontana RJ, et al. Des-γ carboxyprothrombin can differentiate hepatocellular carcinoma from nonmalignant chronic liver disease in American patients. Hepatology. 2003;37:1114–1121
  10. Mita Y, Aoyagi Y, Yanagi M, Suda T, Suzuki Y, Asakura H. The usefulness of determining des-gamma-carboxy prothrombin by sensitive enzyme immunoassay in the early diagnosis of patients with hepatocellular carcinoma. Cancer. 1998;82:1643–1648
  11. Capurro M, Wanless IR, Sherman M, Deboer G, Shi W, Miyoshi E, et al. Glypican-3 (a novel serum and histochemical marker for hepatocellular carcinoma). Gastroenterology. 2003;125:89–97
  12. Yao DF, Huang ZW, Chen SZ, Huang JF, Lu JX, Xiao MB, et al. Diagnosis of hepatocellular carcinoma by quantitative detection of hepatoma-specific bands of serum γ-glutamyltransferase. Am J Clin Pathol. 1998;110:743–749
  13. Cui R, He J, Zhang F, Wang B, Ding H, Shen H, et al. Diagnostic value of protein induced by vitamin K absence (PIVKAII) and hepatoma-specific band of serum gamma-glutamyl transferase (GGTII) as hepatocellular carcinoma markers complementary to α-fetoprotein. Br J Cancer. 2003;88:1878–1882
  14. Chignard N, Beretta L. Proteomics for hepatocellular carcinoma marker discovery. Gastroenterology. 2004;127:S120–S125
  15. Kuick RD, Skolnick MM, Hanash SM, Neel JV. A two-dimensional electrophoresis-related laboratory information processing system (spot matching). Electrophoresis. 1991;12:736–746
  16. Asakawa J, Kuick R, Neel JV, Kodaira M, Satoh C, Hanash SM. Genetic variation detected by quantitative analysis of end-labeled genomic DNA fragments. Proc Natl Acad Sci U S A. 1994;91:9052–9056
  17. Le Naour F, Brichory F, Misek DE, Brechot C, Hanash SM, Beretta L. A distinct repertoire of autoantibodies in hepatocellular carcinoma identified by proteomic analysis. Mol Cell Proteomics. 2002;1:197–203
  18. Kim W, Oe Lim S, Kim JS, Ryu YH, Byeon JY, Kim HJ, et al. Comparison of proteome between hepatitis B virus- and hepatitis C virus-associated hepatocellular carcinoma. Clin Cancer Res. 2003;9:5493–5500
  19. Lim SO, Park SJ, Kim W, Park SG, Kim HJ, Kim YI, et al. Proteome analysis of hepatocellular carcinoma. Biochem Biophys Res Commun. 2002;291:1031–1037
  20. Takashima M, Kuramitsu Y, Yokoyama Y, Iizuka N, Toda T, Sakaida I, et al. Proteomic profiling of heat shock protein 70 family members as biomarkers for hepatitis C virus-related hepatocellular carcinoma. Proteomics. 2003;3:2487–2493
  21. Park KS, Kim H, Kim NG, Cho SY, Choi KH, Seong JK, et al. Proteomic analysis and molecular characterization of tissue ferritin light chain in hepatocellular carcinoma. Hepatology. 2002;35:1459–1466
  22. Anderson NL, Polanski M, Pieper R, Gatlin T, Tirumalai RS, Conrads TP, et al. The human plasma proteome (a nonredundant list developed by combination of four separate sources). Mol Cell Proteomics. 2004;3:311–326
  23. Park KS, Cho SY, Kim H, Paik YK. Proteomic alterations of the variants of human aldehyde dehydrogenase isozymes correlate with hepatocellular carcinoma. Int J Cancer. 2002;97:261–265
  24. Zeindl-Eberhart E, Haraida S, Liebmann S, Jungblut PR, Lamer S, Mayer D, et al. Detection and identification of tumor-associated protein variants in human hepatocellular carcinomas. Hepatology. 2004;39:540–549
  25. Kleizen B, Braakman I. Protein folding and quality control in the endoplasmic reticulum. Curr Opin Cell Biol. 2004;16:343–349
  26. Ellgaard L, Frickel EM. Calnexin, calreticulin, and ERp57 (teammates in glycoprotein folding). Cell Biochem Biophys. 2003;39:223–247
  27. Gelebart P, Opas M, Michalak M. Calreticulin, a Ca2+-binding chaperone of the endoplasmic reticulum. Int J Biochem Cell Biol. 2005;37:260–266
  28. Dhahbi JM, Cao SX, Tillman JB, Mote PL, Madore M, Walford RL, et al. Chaperone-mediated regulation of hepatic protein secretion by caloric restriction. Biochem Biophys Res Commun. 2001;284:335–339
  29. Wang L, Fast DG, Attie AD. The enzymatic and non-enzymatic roles of protein-disulfide isomerase in apolipoprotein B secretion. J Biol Chem. 1997;272:27644–27651
  30. Dorner AJ, Wasley LC, Kaufman RJ. Overexpression of GRP78 mitigates stress induction of glucose regulated proteins and blocks secretion of selective proteins in Chinese hamster ovary cells. EMBO J. 1992;11:1563–1571
  31. Dorner AJ, Krane MG, Kaufman RJ. Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells. Mol Cell Biol. 1988;8:4063–4070
  32. Paulsson K, Wang P. Chaperones and folding of MHC class I molecules in the endoplasmic reticulum. Biochim Biophys Acta. 2003;1641:1–12
  33. Marincola FM, Jaffee EM, Hicklin DJ, Ferrone S. Escape of human solid tumors from T-cell recognition (molecular mechanisms and functional significance). Adv Immunol. 2000;74:181–273
  34. Chang CC, Campoli M, Ferrone S. HLA class I antigen expression in malignant cells (why does it not always correlate with CTL-mediated lysis?). Curr Opin Immunol. 2004;16:644–650
  35. Seliger B, Maeurer MJ, Ferrone S. Antigen-processing machinery breakdown and tumor growth. Immunol Today. 2000;21:455–464
  36. Seliger B, Atkins D, Bock M, Ritz U, Ferrone S, Huber C, et al. Characterization of human lymphocyte antigen class I antigen-processing machinery defects in renal cell carcinoma lesions with special emphasis on transporter-associated with antigen-processing down-regulation. Clin Cancer Res. 2003;9:1721–1727
  37. Seliger B, Ritz U, Abele R, Bock M, Tampe R, Sutter G, et al. Immune escape of melanoma (first evidence of structural alterations in two distinct components of the MHC class I antigen processing pathway). Cancer Res. 2001;61:8647–8650
  38. Seliger B, Wollscheid U, Momburg F, Blankenstein T, Huber C. Characterization of the major histocompatibility complex class I deficiencies in B16 melanoma cells. Cancer Res. 2001;61:1095–1099
  39. Garcia-Lora A, Martinez M, Algarra I, Gaforio JJ, Garrido F. MHC class I-deficient metastatic tumor variants immunoselected by T lymphocytes originate from the coordinated down-regulation of APM components. Int J Cancer. 2003;106:521–527
  40. Ogino T, Bandoh N, Hayashi T, Miyokawa N, Harabuchi Y, Ferrone S. Association of tapasin and HLA class I antigen down-regulation in primary maxillary sinus squamous cell carcinoma lesions with reduced survival of patients. Clin Cancer Res. 2003;9:4043–4051
  41. Dissemond J, Busch M, Kothen T, Mors J, Weimann TK, Lindeke A, et al. Differential down-regulation of endoplasmic reticulum-residing chaperones calnexin and calreticulin in human metastatic melanoma. Cancer Lett. 2004;203:225–231
  42. Hippo Y, Watanabe K, Watanabe A, Midorikawa Y, Yamamoto S, Ihara S, et al. Identification of soluble NH2-terminal fragment of glypican-3 as a serological marker for early-stage hepatocellular carcinoma. Cancer Res. 2004;64:2418–2423
  43. Paradis V, Degos F, Dargere D, Pham N, Belghiti J, Degott C, et al. Identification of a new marker of hepatocellular carcinoma by serum protein profiling of patients with chronic liver diseases. Hepatology. 2004;41:40–47

 Supported by NIH/NIDDK grant RO1 DK066840 and NIH/NCI Early Detection Research Network subcontract (to L.B.).

PII: S0016-5085(06)00737-2

doi: 10.1053/j.gastro.2006.02.058

Gastroenterology
Volume 130, Issue 7 , Pages 2010-2022 , June 2006

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