Gastroenterology
Volume 129, Issue 6 , Pages 1918-1927 , December 2005

A New Method for Isolating Colonocytes From Naturally Evacuated Feces and Its Clinical Application to Colorectal Cancer Diagnosis

  • Hisayuki Matsushita

      Affiliations

    • Investigative Treatment Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa, Japan
  • ,
  • Yasuhiro Matsumura

      Affiliations

    • Investigative Treatment Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa, Japan
    • Corresponding Author InformationAddress requests for reprints to: Yasuhiro Matsumura, MD, PhD, National Cancer Center Research Institute East, 6-5-1 Kashiwanoha, Kashiwa, Chiba 277-8577, Japan. fax: (81) 4-7134-6866.
  • ,
  • Yoshihiro Moriya

      Affiliations

    • Department of Surgery, National Cancer Center Hospital, Tokyo, Japan
  • ,
  • Takayuki Akasu

      Affiliations

    • Department of Surgery, National Cancer Center Hospital, Tokyo, Japan
  • ,
  • Shin Fujita

      Affiliations

    • Department of Surgery, National Cancer Center Hospital, Tokyo, Japan
  • ,
  • Seiichiro Yamamoto

      Affiliations

    • Department of Surgery, National Cancer Center Hospital, Tokyo, Japan
  • ,
  • Shigeki Onouchi

      Affiliations

    • Department of Surgery, National Cancer Center Hospital, Tokyo, Japan
  • ,
  • Norio Saito

      Affiliations

    • Department of Surgery, National Cancer Center Hospital East, Kashiwa, Japan
  • ,
  • Masanori Sugito

      Affiliations

    • Department of Surgery, National Cancer Center Hospital East, Kashiwa, Japan
  • ,
  • Masaaki Ito

      Affiliations

    • Department of Surgery, National Cancer Center Hospital East, Kashiwa, Japan
  • ,
  • Takahiro Kozu

      Affiliations

    • Cancer Screening Division, National Cancer Center Research Center for Cancer Prevention and Screening, Tokyo, Japan
  • ,
  • Takashi Minowa

      Affiliations

    • Hitachi, Ltd., Life Science Group, Saitama, Japan
  • ,
  • Sayuri Nomura

      Affiliations

    • Hitachi, Ltd., Life Science Group, Saitama, Japan
  • ,
  • Hiroyuki Tsunoda

      Affiliations

    • Hitachi, Ltd., Advanced Research Laboratory, Tokyo, Japan
  • ,
  • Tadao Kakizoe

      Affiliations

    • National Cancer Center, Tokyo, Japan

Received 24 May 2005 ,Accepted 31 August 2005.

References 

  1. The Editorial Board of the Cancer Statistics in Japan . Cancer statistics in Japan— . 2003; http://www.fpcr.or.jp. Accessed 2003
  2. Mandel JS, Bond JH, Church TR, Snover DC, Bradley GM, Schuman LM, et al. Reducing mortality from colorectal cancer by screening for fecal occult blood . N Engl J Med . 1993;328:1365–1371
  3. Hardcastle JD, Chamberlain JO, Robinson MH, Moss SM, Amar SS, Balfour TW, et al. Randomised controlled trial of faecal-occult-blood screening for colorectal cancer . Lancet . 1996;348:1472–1477
  4. Kronborg O , Fenger C , Olsen J , Jorgensen OD , Sondergaard O . Randomized study of screening for colorectal cancer with faecal-occult-blood test . Lancet . 1996;348:1467–1471
  5. Towler B , Irwig L , Glasziou P , Kewenter J , Weller D , Silagy C . A systematic review of the effects of screening for colorectal cancer using the faecal occult blood test, hemoccult . BMJ . 1998;317:559–565
  6. Winawer S, Fletcher R, Rex D, Bond J, Burt R, Ferrucci J, et al. Colorectal cancer screening and surveillance (clinical guidelines and rationale—update based on new evidence) . Gastroenterology . 2003;124:544–560
  7. Mandel JS, Church TR, Bond JH, Ederer F, Geisser MS, Mongin SJ, et al. The effect of fecal occult-blood screening on the incidence of colorectal cancer . N Engl J Med . 2000;343:1603–1607
  8. Sidransky D, Tokino T, Hamilton SR, Kinzler KW, Levin B, Frost P, et al. Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors . Science . 1992;256:102–105
  9. Hasegawa Y, Takeda S, Ichii S, Koizumi K, Maruyama M, Fujii A, et al  Detection of K-ras mutations in DNAs isolated from feces of patients with colorectal tumors by mutant-allele-specific amplification (MASA) . Oncogene . 1995;10:1441–1445
  10. Smith-Ravin J , England J , Talbot IC , Bodmer W . Detection of c-Ki-ras mutations in faecal samples from sporadic colorectal cancer patients . Gut . 1995;36:81–86
  11. Eguchi S , Kohara N , Komuta K , Kanematsu T . Mutations of the p53 gene in the stool of patients with resectable colorectal cancer . Cancer . 1996;77:1707–1710
  12. Nollau P , Moser C , Weinland G , Wagener C . Detection of K-ras mutations in stools of patients with colorectal cancer by mutant-enriched PCR . Int J Cancer . 1996;66:332–336
  13. Ratto C, Flamini G, Sofo L, Nucera P, Ippoliti M, Curigliano G, et al. Detection of oncogene mutation from neoplastic colonic cells exfoliated in feces . Dis Colon Rectum . 1996;39:1238–1244
  14. Deuter R , Muller O . Detection of APC mutations in stool DNA of patients with colorectal cancer by HD-PCR . Hum Mutat . 1998;11:84–89
  15. Ahlquist DA, Skoletsky JE, Boynton KA, Harrington JJ, Mahoney DW, Pierceall WE, et al. Colorectal cancer screening by detection of altered human DNA in stool (feasibility of a multitarget assay panel) . Gastroenterology . 2000;119:1219–1227
  16. Dong SM, Traverso G, Johnson C, Geng L, Favis R, Boynton K, et al. Detecting colorectal cancer in stool with the use of multiple genetic targets . J Natl Cancer Inst . 2001;93:858–865
  17. Rengucci C , Maiolo P , Saragoni L , Zoli W , Amadori D , Calistri D . Multiple detection of genetic alterations in tumors and stool . Clin Cancer Res . 2001;7:590–593
  18. Traverso G, Shuber A, Olsson L, Levin B, Johnson C, Hamilton SR, et al. Detection of proximal colorectal cancers through analysis of faecal DNA . Lancet . 2002;359:403–404
  19. Traverso G, Shuber A, Levin B, Johnson C, Olsson L, Schoetz DJ, et al. Detection of APC mutations in fecal DNA from patients with colorectal tumors . N Engl J Med . 2002;346:311–320
  20. Boynton KA , Summerhayes IC , Ahlquist DA , Shuber AP . DNA integrity as a potential marker for stool-based detection of colorectal cancer . Clin Chem . 2003;49:1058–1065
  21. Yamao T, Matsumura Y, Shimada Y, Moriya Y, Sugihara K, Akasu T, et al. Abnormal expression of CD44 variants in the exfoliated cells in the feces of patients with colorectal cancer . Gastroenterology . 1998;114:1196–1205
  22. Davies RJ, Freeman A, Morris LS, Bingham S, Dilworth S, Scott I, et al. Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool . Lancet . 2002;359:1917–1919
  23. Winter MJ , Nagtegaal ID , van Krieken JH , Litvinov SV . The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology . Am J Pathol . 2003;163:2139–2148
  24. Balzar M , Prins FA , Bakker HAM , Fleuren GJ , Warnaar SO , Litvinov SV . The structural analysis of adhesions mediated by Ep-CAM . Exp Cell Res . 1999;246:108–121
  25. Salem RR, Wolf BC, Sears HF, Lavin PT, Ravikumar TS, DeCoste D, et al. Expression of colorectal carcinoma-associated antigens in colonic polyps . J Surg Res . 1993;55:249–255
  26. Iyengar V , Albaugh GP , Lohani A , Nair PP . Human stools as a source of viable colonic epithelial cells . FASEB J . 1991;5:2856–2859
  27. Davidson LA , Lupton JR , Miskovsky E , Fields AP , Chapkin RS . Quantification of human intestinal gene expression profiles using exfoliated colonocytes (a pilot study) . Biomarkers . 2003;8:51–61
  28. Imperiale TF , Ransohoff DF , Itzkowitz SH , Turnbull BA , Ross ME . Fecal DNA versus fecal occult blood for colorectal cancer screening in an average-risk population . N Engl J Med . 2004;351:2704–2714
  29. Matsumura Y , Tarin D . Significance of CD44 gene products for cancer diagnosis and disease evaluation . Lancet . 1992;340:1053–1058
  30. Matsumura Y , Hanbury D , Smith J , Tarin D . Non-invasive detection of malignancy by identification of unusual CD44 gene activity in exfoliated cancer cells . BMJ . 1994;308:619–624

 Supported in part by a grant for research on advanced medical technology from the Ministry of Health, Welfare, and Labor and a research and development project of the Industrial Science and Technology Program supported by the New Energy and Industrial Technology Development Organization (NEDO) of Japan.

PII: S0016-5085(05)02018-4

doi: 10.1053/j.gastro.2005.10.007

Gastroenterology
Volume 129, Issue 6 , Pages 1918-1927 , December 2005