Gastroenterology
Volume 128, Issue 4, Supplement 1 , Pages S38-S46 , April 2005

The diagnostic accuracy of serologic tests for celiac disease: A systematic review

  • Alaa Rostom

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • Corresponding Author InformationAddress requests for reprints to: Alaa Rostom, MD, MSc, FRCPC, Gastrointestinal Clinical Research Unit, Division of Gastroenterology, University of Ottawa, Ottawa Hospital-Civic Campus, 1053 Carling Ave., Ottawa, Ontario, Canada K1Y 4E9.
    • ,
  • Catherine Dubé

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • Ann Cranney

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • Navaaz Saloojee

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • Richmond Sy

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • Chantelle Garritty

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada
    • ,
  • Margaret Sampson

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada
    • ,
  • Li Zhang

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada
    • ,
  • Fatemeh Yazdi

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada
    • ,
  • Vasil Mamaladze

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada
    • ,
  • Irene Pan

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada
    • ,
  • Joanne MacNeil

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • David Mack

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • Dilip Patel

      Affiliations

    • Gastrointestinal Clinical Research Unit, University of Ottawa, Ottawa, Canada
    • ,
  • David Moher

      Affiliations

    • Chalmers Research Group, University of Ottawa, Ottawa, Canada

References 

  1. Fasano A, Berti I, Gerarduzzi T, Not T, Colletti RB, Drago S, et al. Prevalence of celiac disease in at-risk and not-at-risk groups in the United States (a large multicenter study) . Arch Intern Med . 2003;163:286–292
  2. Tommasini A, Not T, Kiren V, Baldas V, Santon D, Trevisiol C, et al. Mass screening for coeliac disease using antihuman transglutaminase antibody assay . Arch Dis Child . 2004;89:512–515
  3. Whiting P , Rutjes AW , Reitsma JB , Bossuyt PM , Kleijnen J . The development of QUADAS (a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews) . BMC Med Res Methodol . 2003;3:25
  4. Moses LE , Shapiro D , Littenberg B . Combining independent studies of a diagnostic test into a summary ROC curve (data-analytic approaches and some additional considerations) . Stat Med . 1993;12:1293–1316
  5. Littenberg B , Moses LE . Estimating diagnostic accuracy from multiple conflicting reports (a new meta-analytic method) . Med Decis Making . 1993;13:313–321
  6. Altuntas B , Kansu A , Ensari A , Girgin N . Celiac disease in Turkish short-statured children and the value of antigliadin antibody in diagnosis . Acta Paediatr Jpn . 1998;40:457–460
  7. Ascher H , Hahn-Zoric M , Hanson LA , Kilander AF , Nilsson LA , Tlaskalova H . Value of serologic markers for clinical diagnosis and population studies of coeliac disease . Scand J Gastroenterol . 1996;31:61–67
  8. Ascher H , Lanner A , Kristiansson B . A new laboratory kit for anti-gliadin IgA at diagnosis and follow-up of childhood celiac disease . J Pediatr Gastroenterol Nutr . 1990;10:443–450
  9. Bahia M , Rabello A , Brasileiro FG , Penna FJ . Serum antigliadin antibody levels as a screening criterion before jejunal biopsy indication for celiac disease in a developing country. (Revista Brasileira De Pesquisas Medicas E Biologicas/Sociedade Brasileira De Biofisica Et Al) . Braz J Med Biologic Res . 2001;34:1415–1420
  10. Bardella MT , Trovato C , Cesana BM , Pagliari C , Gebbia C , Peracchi M . Serological markers for coeliac disease (is it time to change?) . Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver. Dig Liver Dis . 2001;33:426–431
  11. Berger R , Schmidt G . Evaluation of six anti-gliadin antibody assays . J Immunol Meth . 1996;191:77–86
  12. Biagi F , Pezzimenti D , Campanella J , Vadacca GB , Corazza GR . Endomysial and tissue transglutaminase antibodies in coeliac sera (a comparison not influenced by previous serologic testing) . Scand J Gastroenterol . 2001;36:955–958
  13. Bode S , Gudmand-Hoyer E . Evaluation of the gliadin antibody test for diagnosing coeliac disease . Scand J Gastroenterol . 1994;29:148–152
  14. Bode S , Weile B , Krasilnikoff PA , Gudmand-Hoyer E . The diagnostic value of the gliadin antibody test in celiac disease in children (a prospective study) . J Pediatr Gastroenterol Nutr . 1993;17:260–264
  15. Bonamico M, Tiberti C, Picarelli A, Mariani P, Rossi D, Cipolletta E, et al. Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease . Am J Gastroenterol . 2001;96:1536–1540
  16. Bottaro G , Volta U , Spina M , Rotolo N , Sciacca A , Musumeci S . Antibody pattern in childhood celiac disease . J Pediatr Gastroenterol Nutr . 1997;24:559–562
  17. Carroccio A, Iacono G, D’Amico D, Cavataio F, Teresi S, Caruso C, et al. Production of anti-endomysial antibodies in cultured duodenal mucosa (usefulness in coeliac disease diagnosis) . Scand J Gastroenterol . 2002;37:32–38
  18. Carroccio A, Iacono G, Montalto G, Cavataio F, Soresi M, Kazmierska I, et al. Immunologic and absorptive tests in celiac disease (can they replace intestinal biopsies?) . Scand J Gastroenterol . 1993;28:673–676
  19. Carroccio A, Vitale G, Di Prima L, Chifari N, Napoli S, La Russa C, et al. Comparison of anti-transglutaminase ELISAs and an anti-endomysial antibody assay in the diagnosis of celiac disease (a prospective study) . Clin Chem . 2002;48:1546–1550
  20. Cataldo F , Lio D , Marino V , Picarelli A , Ventura A , Corazza GR  Working Groups on Celiac Disease of SIGEP and Club del Tenue . IgG(1) antiendomysium and IgG antitissue transglutaminase (anti-tTG) antibodies in coeliac patients with selective IgA deficiency . Gut . 2000;47:366–369
  21. Chan AW , Butzner JD , McKenna R , Fritzler MJ . Tissue transglutaminase enzyme-linked immunosorbent assay as a screening test for celiac disease in pediatric patients . Pediatrics . 2001;107:E8
  22. Chartrand LJ , Agulnik J , Vanounou T , Russo PA , Baehler P , Seidman EG . Effectiveness of antigliadin antibodies as a screening test for celiac disease in children . CMAJ . 1997;157:527–533
  23. Chirdo FG, Rumbo M, Carabajal P, Castagnino N, Mavromatopulos E, Cirincione V, et al. Analysis of anti-gliadin antibodies by immunoblot analysis and enzyme-linked immunosorbent assay using gliadin fractions as antigens . J Pediatr Gastroenterol Nutr . 1999;29:171–177
  24. Chirdo FG, Rumbo M, Carabajal P, Mavromatopulos E, Castagnino N, Anon MC, et al. Determination of anti-omega-gliadin antibodies in serologic tests for coeliac disease . Scand J Gastroenterol . 2000;35:508–516
  25. Dahele AV , Aldhous MC , Humphreys K , Ghosh S . Serum IgA tissue transglutaminase antibodies in coeliac disease and other gastrointestinal diseases . Q J Med . 2001;94:195–205
  26. Di Leo M , Weisz G , Ansaldi BN . Serum and salivary antiendomysium antibodies in the screening of coeliac disease . Panminerva Medica . 1999;41:68–71
  27. Dickey W , McMillan SA , Hughes DF . Sensitivity of serum tissue transglutaminase antibodies for endomysial antibody positive and negative coeliac disease . Scand J Gastroenterol . 2001;36:511–514
  28. Falth-Magnusson K , Jansson G , Stenhammar L , Magnusson KE . Serum food antibodies analyzed by enzyme-linked immunosorbent assay (ELISA) and diffusion-in-gel (DIG)-ELISA methods in children with and without celiac disease . J Pediatr Gastroenterol Nutr . 1994;18:56–62
  29. Gillett HR , Freeman HJ . Comparison of IgA endomysium antibody and IgA tissue transglutaminase antibody in celiac disease . Can J Gastroenterol . 2000;14:668–671
  30. Gonczi J , Skerritt JH , Mitchell JD . A reliable screening test for coeliac disease (enzyme-linked immunosorbent assay to detect anti-gliadin antibodies in serum) . Aust N Z J Med . 1991;21:723–731
  31. Hallstrom O . Comparison of IgA-class reticulin and endomysium antibodies in coeliac disease and dermatitis herpetiformis . Gut . 1989;30:1225–1232
  32. Hansson T, Dahlbom I, Hall J, Holtz A, Elfman L, Dannaeus A, et al. Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease . J Pediatr Gastroenterol Nutr . 2000;30:379–384
  33. Iltanen S , Rantala I , Laippala P , Holm K , Partanen J , Maki M . Expression of HSP-65 in jejunal epithelial cells in patients clinically suspected of coeliac disease . Autoimmunity . 1999;31:125–132
  34. Kaukinen K, Turjanmaa K, Maki M, Partanen J, Venalainen R, Reunala T, et al. Intolerance to cereals is not specific for coeliac disease . Scand J Gastroenterol . 2000;35:942–946
  35. Kolho KL , Savilahti E . IgA endomysium antibodies on human umbilical cord (an excellent diagnostic tool for celiac disease in childhood) . J Pediatr Gastroenterol Nutr . 1997;24:563–567
  36. Kumar V , Lerner A , Valeski JE , Beutner EH , Chorzelski TP , Rossi T . Endomysial antibodies in the diagnosis of celiac disease and the effect of gluten on antibody titers . Immunol Invest . 1989;18:533–544
  37. Ladinser B , Rossipal E , Pittschieler K . Endomysium antibodies in coeliac disease (an improved method) . Gut . 1994;35:776–778
  38. Lerner A , Kumar V , Iancu TC . Immunological diagnosis of childhood coeliac disease (comparison between antigliadin, antireticulin and antiendomysial antibodies) . Clin Exp Immunol . 1994;95:78–82
  39. Lindberg T, Nilsson LA, Borulf S, Cavell B, Fallstrom SP, Jansson U, et al. Serum IgA and IgG gliadin antibodies and small intestinal mucosal damage in children . J Pediatr Gastroenterol Nutr . 1985;4:917–922
  40. Lindquist BL , Rogozinski T , Moi H , Danielsson D , Olcen P . Endomysium and gliadin IgA antibodies in children with coeliac disease . Scand J Gastroenterol . 1994;29:452–456
  41. Maki M, Holm K, Lipsanen V, Hallstrom O, Viander M, Collin P, et al. Serological markers and HLA genes among healthy first-degree relatives of patients with coeliac disease . Lancet . 1991;338:1350–1353
  42. McMillan SA , Haughton DJ , Biggart JD , Edgar JD , Porter KG , McNeill TA . Predictive value for coeliac disease of antibodies to gliadin, endomysium, and jejunum in patients attending for jejunal biopsy . BMJ (Clinical research ed) . 1991;303:1163–1165
  43. Meini A , Pillan NM , Villanacci V , Monafo V , Ugazio AG , Plebani A . Prevalence and diagnosis of celiac disease in IgA-deficient children . Annals of Allergy Asthma Immunol . 1996;77:333–336
  44. Pacht A , Sinai N , Hornstein L , Kumar V , Ish-Shalom N , Lerner A . The diagnostic reliability of anti-endomysial antibody in celiac disease (the north Israel experience) . Isr J Med Sci . 1995;31:218–220
  45. Picarelli A, Sabbatella L, Di Tola M, Gabrielli F, Greco R, Di Cello T, et al. Celiac disease diagnosis in misdiagnosed children . Pediatr Res . 2000;48:590–592
  46. Poddar U , Thapa BR , Nain CK , Prasad A , Singh K . Celiac disease in India (are they true cases of celiac disease?) . J Pediatr Gastroenterol Nutr . 2002;35:508–512
  47. Rich EJ , Christie DL . Anti-gliadin antibody panel and xylose absorption test in screening for celiac disease . J Pediatr Gastroenterol Nutr . 1990;10:174–178
  48. Russo PA , Chartrand LJ , Seidman E . Comparative analysis of serologic screening tests for the initial diagnosis of celiac disease . Pediatrics . 1999;104:75–78
  49. Salmaso C, Ocmant A, Pesce G, Altrinetti V, Montagna P, Descalzi D, et al. Comparison of ELISA for tissue transglutaminase autoantibodies with antiendomysium antibodies in pediatric and adult patients with celiac disease . Allergy . 2001;56:544–547
  50. Sategna-Guidetti C , Grosso S , Bruno M , Grosso SB . Comparison of serum anti-gliadin, anti-endomysium, and anti-jejunum antibodies in adult celiac sprue . J Clin Gastroenterol . 1995;20:17–21
  51. Sblattero D, Berti I, Trevisiol C, Marzari R, Tommasini A, Bradbury A, et al. Human recombinant tissue transglutaminase ELISA (an innovative diagnostic assay for celiac disease) . Am J Gastroenterol . 2000;95:1253–1257
  52. Sulkanen S , Collin P , Laurila K , Maki M . IgA- and IgG-class antihuman umbilical cord antibody tests in adult coeliac disease . Scand J Gastroenterol . 1998;33:251–254
  53. Sulkanen S, Halttunen T, Laurila K, Kolho KL, Korponay-Szabo IR, Sarnesto A, et al. Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease . Gastroenterology . 1998;115:1322–1328
  54. Tesei N, Sugai E, Vazquez H, Smecuol E, Niveloni S, Mazure R, et al. Antibodies to human recombinant tissue transglutaminase may detect coeliac disease patients undiagnosed by endomysial antibodies . Aliment Pharmacol Ther . 2003;17:1415–1423
  55. Troncone R, Maurano F, Rossi M, Micillo M, Greco L, Auricchio R, et al. IgA antibodies to tissue transglutaminase (an effective diagnostic test for celiac disease) . J Pediatr . 1999;134:166–171
  56. Valdimarsson T , Franzen L , Grodzinsky E , Skogh T , Strom M . Is small bowel biopsy necessary in adults with suspected celiac disease and IgA anti-endomysium antibodies? 100% positive predictive value for celiac disease in adults . Dig Dis Sci . 1996;41:83–87
  57. Valentini RA , Andreani ML , Corazza GR , Gasbarrini G . IgA endomysium antibody (a valuable tool in the screening of coeliac disease but not its follow-up) . Ital J Gastroenterol . 1994;26:279–282
  58. Vitoria JC , Arrieta A , Ortiz L , Ayesta A . Antibodies to human tissue transglutaminase for the diagnosis of celiac disease . J Pediatr Gastroenterol Nutr . 2001;33:349–350
  59. Vogelsang H, Genser D, Wyatt J, Lochs H, Ferenci P, Granditsch G, et al. Screening for celiac disease (a prospective study on the value of noninvasive tests) . Am J Gastroenterol . 1995;90:394–398
  60. Volta U , Molinaro N , De Franceschi L , Fratangelo D , Bianchi FB . IgA anti-endomysial antibodies on human umbilical cord tissue for celiac disease screening. Save both money and monkeys . Dig Dis Sci . 1995;40:1902–1905
  61. Whelan A , Willoughby R , Weir D . Human umbilical vein endothelial cells (a new easily available source of endomysial antigens) . Eur J Gastroenterol Hepatol . 1996;8:961–966
  62. Wolters V, Vooijs-Moulaert A, Burger H, Brooimans R, De Schryver J, Rijkers G, et al. Human tissue transglutaminase enzyme linked immunosorbent assay outperforms both the guinea pig based tissue transglutaminase assay and anti-endomysium antibodies when screening for coeliac disease . Eur J Pediatr . 2002;161:284–287
  63. Artan R . Antigliadin antibody measurement as a screening test for childhood coeliac disease . Int Med J . 1998;5:209–212
  64. Dahele A , Kingstone K , Bode J , Anderson D , Ghosh S . Anti-endomysial antibody negative celiac disease (does additional serologic testing help?) . Dig Dis Sci . 2001;46:214–221
  65. Brandborg LL , Goldberg SB , Breidenbach WC . Human coccidiosis—a possible cause of malabsorption . N Engl J Med . 1970;283:1306–1313
  66. Hazama H, Omagari K, Masuda J, Ohba K, Kinoshita H, Matsuo I, et al. Automated enzymatic mitochondrial antibody assay for the diagnosis of primary biliary cirrhosis (applications of a routine diagnostic tool for the detection of antimitochondrial antibodies) . J Gastroenterol Hepatol . 2002;17:316–323
  67. Rostami K , Kerckhaert J , Tiemessen R , von Blomberg BM , Meijer JW , Mulder CJ . Sensitivity of antiendomysium and antigliadin antibodies in untreated celiac disease (disappointing in clinical practice) . Am J Gastroenterol . 1999;94:888–894
  68. Tursi A , Brandimarte G , Giorgetti GM . Prevalence of antitissue transglutaminase antibodies in different degrees of intestinal damage in celiac disease . J Clin Gastroenterol . 2003;36:219–221
  69. Tursi A , Brandimarte G , Giorgetti GM . Sorbitol H2-breath test versus anti-endomysium antibodies for the diagnosis of subclinical/silent coeliac disease . Scand J Gastroenterol . 2001;36:1170–1172

PII: S0016-5085(05)00197-6

doi: 10.1053/j.gastro.2005.02.028

Gastroenterology
Volume 128, Issue 4, Supplement 1 , Pages S38-S46 , April 2005

Article Tools

* Image Usage & Resolution