Gastroenterology
Volume 137, Issue 5 , Pages 1584-1592.e1 , November 2009

Feasibility and Reliability of Pancreatic Cancer Staging Using Fiberoptic Confocal Fluorescence Microscopy in Rats

  • Mihaela Ignat

      Affiliations

    • IRCAD, Strasbourg, France
    • University of Strasbourg, Strasbourg, France
    • ,
  • Marc Aprahamian

      Affiliations

    • Inserm U701, IRCAD, Strasbourg, France
    • ,
  • Veronique Lindner

      Affiliations

    • Centre Hospitalier Emile Muller, Service de Pathologie, Mulhouse, France
    • ,
  • Anaïs Altmeyer

      Affiliations

    • IRCAD, Strasbourg, France
    • ,
  • Silvana Perretta

      Affiliations

    • IRCAD, Strasbourg, France
    • University of Strasbourg, Strasbourg, France
    • Hôpitaux Universitaires, Chirurgie Digestive et Endocrinienne, NHC, Strasbourg, France
    • ,
  • Bernard Dallemagne

      Affiliations

    • IRCAD, Strasbourg, France
    • University of Strasbourg, Strasbourg, France
    • Hôpitaux Universitaires, Chirurgie Digestive et Endocrinienne, NHC, Strasbourg, France
    • ,
  • Didier Mutter

      Affiliations

    • IRCAD, Strasbourg, France
    • University of Strasbourg, Strasbourg, France
    • Hôpitaux Universitaires, Chirurgie Digestive et Endocrinienne, NHC, Strasbourg, France
    • ,
  • Jacques Marescaux

      Affiliations

    • IRCAD, Strasbourg, France
    • University of Strasbourg, Strasbourg, France
    • Hôpitaux Universitaires, Chirurgie Digestive et Endocrinienne, NHC, Strasbourg, France
    • Corresponding Author InformationReprint requests Address requests for reprints to: Jacques Marescaux, MD, FRCS, FACS, IRCAD, 1 place de l'hôpital, 67091 Strasbourg, France. fax: (33) 388 119 096

Received 12 August 2008 ,Accepted 10 July 2009.

References 

  1. Brooks AD, Mallis MJ, Brennan MF, et al. The value of laparoscopy in the management of ampullary, duodenal, and distal bile duct tumors. J Gastrointest Surg. 2002;6:139–145
  2. Menack MJ, Spitz JD, Arregui ME, et al. Staging of pancreatic and ampullary cancers for resectability using laparoscopy with laparoscopic ultrasound. Surg Endosc. 2001;15:1129–1134
  3. Doi R, Kami K, Ito D, et al. Prognostic implication of para-aortic lymph node metastasis in resectable pancreatic cancer. World J Surg. 2007;31:147–154
  4. Shimada K, Sakamoto Y, Sano T, et al. The role of paraaortic lymph node involvement on early recurrence and survival after macroscopic curative resection with extended lymphadenectomy for pancreatic carcinoma. J Am Coll Surg. 2006;203:345–352
  5. Ishikawa O, Ohigashi H, Sasaki Y, et al. Practical grouping of positive lymph nodes in pancreatic head cancer treated by an extended pancreatectomy. Surgery. 1997;121:244–249
  6. Yeo CJ, Cameron JL, Lillemoe KD, et al. Pancreaticoduodenectomy with or without distal gastrectomy and extended retroperitoneal lymphadenectomy for periampullary adenocarcinoma, part 2: randomized controlled trial evaluating survival, morbidity, and mortality. Ann Surg. 2002;236:355–366
  7. Cioc AM, Ellison EC, Proca DM, et al. Frozen section diagnosis of pancreatic lesions. Arch Pathol Lab Med. 2002;126:1169–1173
  8. Scheunemann P, Stoecklein NH, Rehders A, et al. Occult tumor cells in lymph nodes as a predictor for tumor relapse in pancreatic adenocarcinoma. Langenbecks Arch Surg. 2008;393:359–365
  9. Kocher HM, Sohail M, Benjamin IS, et al. Technical limitations of lymph node mapping in pancreatic cancer. Eur J Surg Oncol. 2007;33:887–891
  10. Mauna Kea Technologies (Our Technologies). http://www.maunakeatech.com/us/techno/ourtechnoAccessed April 6, 2009.
  11. Laemmel E, Genet M, Le Goualher G, et al. Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation (A comparison with intravital microscopy). J Vasc Res. 2004;41:400–411
  12. Al-Gubory KH, Houdebine LM. In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy. Eur J Cell Biol. 2006;85:837–845
  13. Thiberville L, Moreno-Swirc S, Vercauteren T, et al. In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy. Am J Respir Crit Care Med. 2007;175:22–31
  14. Becker V, Vercauteren T, von Weyhern CH, et al. High-resolution miniprobe-based confocal microscopy in combination with video mosaicing. Gastrointest Endosc. 2007;66:1001–1007
  15. Soltesz EG, Kim S, Laurence RG, et al. Intraoperative sentinel lymph node mapping of the lung using near-infrared fluorescent quantum dots. Ann Thorac Surg. 2005;79:269–277
  16. Jean F, Bourg-Heckly G, Viellerobe B. Fibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis. Optics Express. 2007;15:4008–4017
  17. Weissleder R, Tung CH, Mahmood U, et al. In vivo imaging of tumors with protease-activated near-infrared fluorescent probes. Nat Biotechnol. 1999;17:375–378
  18. Marecos E, Weissleder R, Bogdanov A. Antibody-mediated versus nontargeted delivery in a human small cell lung carcinoma model. Bioconjug Chem. 1998;9:184–191
  19. Pettengill OS, Faris RA, Bell RH, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am J Pathol. 1993;143:292–303
  20. Mutter D, Hajri A, Tassetti V, et al. Increased tumor growth and spread after laparoscopy vs laparotomy: influence of tumor manipulation in a rat model. Surg Endosc. 1999;13:365–370
  21. Clancy TE, Ashley SW. Pancreaticoduodenectomy (Whipple operation). Oncol Clin N Am. 2005;14:533–552
  22. Lim JE, Chien MW, Earle CC. Prognostic factors following curative resection for pancreatic adenocarcinoma: a population-based, linked database analysis of 396 patients. Ann Surg. 2003;237:74–85
  23. Govindarajan A, Tan JC, Baxter NN, et al. Variations in surgical treatment and outcomes of patients with pancreatic cancer: a population-based study. Ann Surg Oncol. 2008;15:175–185
  24. Yeo CJ, Cameron JL, Lillemoe KD, et al. Pancreaticoduodenectomy for cancer of the head of the pancreas (201 patients). Ann Surg. 1995;221:721–733
  25. Berger AC, Watson JC, Ross EA, et al. The metastatic/examined lymph node ratio is an important prognostic factor after pancreaticoduodenectomy for pancreatic adenocarcinoma. Am Surg. 2004;70:235–240
  26. Pawlik TM, Gleisner AL, Cameron JL, et al. Prognostic relevance of lymph node ratio following pancreaticoduodenectomy for pancreatic cancer. Surgery. 2007;141:610–618
  27. Yoshida T, Matsumoto T, Sasaki A, et al. Outcome of paraaortic node-positive pancreatic head and bile duct adenocarcinoma. Am J Surg. 2004;187:736–740
  28. Bley TA, Uhl M, Simon P, et al. Diagnostic accuracy of MRI for preoperative staging of pancreatic carcinoma: tendency for understaging. In Vivo. 2005;19:983–987
  29. Pauls S, Sokiranski R, Schwarz M, et al. Value of spiral CT and MRI (1.5 T) in preoperative diagnosis of tumors of the head of the pancreas. Rontgenpraxis. 2003;55:3–15
  30. Soriano A, Castells A, Ayuso C, et al. Preoperative staging and tumor resectability assessment of pancreatic cancer: prospective study comparing endoscopic ultrasonography, helical computed tomography, magnetic resonance imaging, and angiography. Am J Gastroenterol. 2004;99:492–501
  31. Schwarz M, Pauls S, Sokiranski R, et al. Is a preoperative multidiagnostic approach to predict surgical resectability of periampullary tumors still effective?. Am J Surg. 2001;182:243–249
  32. Wong T, Kocher H, Kane P, et al. The role of endoscopic ultrasound and helical computed tomography in pancreatic masses: results from a UK centre. Gut. 2004;48(Suppl):A65
  33. Lemke AJ, Niehues SM, Hosten N, et al. Retrospective digital image fusion of multidetector CT and 18F-FDG PET: clinical value in pancreatic lesions—a prospective study with 104 patients. J Nucl Med. 2004;45:1279–1286
  34. Cordera F, Arciero CA, Li T, et al. Significance of common hepatic artery lymph node metastases during pancreaticoduodenectomy for pancreatic head adenocarcinoma. Ann Surg Oncol. 2007;14:2330–2336
  35. Kayahara M, Nagakawa T, Ohta T, et al. Analysis of paraaortic lymph node involvement in pancreatic carcinoma: a significant indication for surgery?. Cancer. 1999;85:583–590
  36. Chen SL, Iddings DM, Scheri RP, et al. Lymphatic mapping and sentinel node analysis: current concepts and applications. CA Cancer J Clin. 2006;56:292–309
  37. Nagakawa T, Kobayashi H, Ueno K, et al. Clinical study of lymphatic flow to the paraaortic lymph nodes in carcinoma of the head of the pancreas. Cancer. 1994;73:1155–1162
  38. Kayahara M, Nakagawara H, Kitagawa H, et al. The nature of neural invasion by pancreatic cancer. Pancreas. 2007;35:218–223
  39. Nathanson SD. Insights into the mechanisms of lymph node metastasis. Cancer. 2003;98:413–423

 Conflicts of interest The authors disclose no conflicts.

 Funding Supported by Association pour la Recherche sur le Cancer, project P08/2-02/07/08 (to I.M.).

PII: S0016-5085(09)01244-X

doi: 10.1053/j.gastro.2009.07.045

Gastroenterology
Volume 137, Issue 5 , Pages 1584-1592.e1 , November 2009

Article Tools

* Image Usage & Resolution