Gastroenterology
Volume 97, Issue 1 , Pages 29-37 , July 1989

Studies of intestinal lymphoid tissue. XII. Epithelial lymphocyte and mucosal responses to rectal gluten challenge in celiac sprue

  • Duncan E. Loft

      Affiliations

    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom
    • Dr. Loft was the recipient of an AGA International Travel Fellowship.
    • ,
  • Michael N. Marsh

      Affiliations

    • Corresponding Author InformationAddress requests for reprints to: Dr. Michael N. Marsh, University Department of Medicine, Clinical Sciences Building, Hope Hospital, Eccles Old Road, Salford, Manchester M6 8HD, United Kingdom.
    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom
    • Dr. Marsh and Dr. Sandle are supported by the Medical Research Council of Great Britain.
    • ,
  • Geoffrey I. Sandle

      Affiliations

    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom
    • Dr. Marsh and Dr. Sandle are supported by the Medical Research Council of Great Britain.
    • ,
  • Peter T. Crowe

      Affiliations

    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom
    • ,
  • Victor Garner

      Affiliations

    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom
    • ,
  • Derek Gordon

      Affiliations

    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom
    • ,
  • Rosemary Baker

      Affiliations

    • Department of Medicine, University of Manchester School of Medicine Manchester, United Kingdom
    • The John Dalton Institute of Technology, Manchester, United Kingdom
    • The Laboratory of Computational Science, University of Salford, Salford, United Kingdom

Received 8 June 1988 ,Accepted 30 January 1989.

References 

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  2. Dobbins WO, Rubin CE. Studies of the rectal mucosa in coeliac sprue. Gastroenterology. 1964;47:471–479
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  5. Marsh MN. Studies of intestinal lymphoid tissue. IV—The predictive value of raised mitotic indices among jejunal epithelial lymphocytes in the diagnosis of gluten-sensitive enteropathy. J Clin Pathol. 1983;35:517–525
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  7. Niazi NM, Leigh RJ, Crowe P, Marsh MN. Morphometric analysis of small intestinal mucosa. I—Methodology, epithelial volume compartments and enumeration of inter-epithelial space lymphocytes. Virchows Arch [B]. 1984;404:49–60
  8. Dhesi I, Marsh MN, Kelly C, Crowe P. Morphometric analysis of small intestinal mucosa. II—Determination of lamina propria volumes; plasma cell and neutrophil populations within control and celiac disease mucosae. Virchows Arch [B]. 1984;403:173–180
  9. Marsh MN, Hinde J. Morphometric analysis of small intestinal mucosa. III—The quantitation of crypt epithelial volumes and lymphoid cell infiltrates, with reference to celiac sprue mucosae. Virchows Arch [B]. 1986;409:11–22
  10. Giger H, Riedwyl H. Bestimmung der Grössenverteilung von Kugeln aus Schnitt Kreisadien. Biometr Zeitschr. 1970;12:156–165
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  13. Marsh MN, Hinde J. Inflammatory component of celiac sprue mucosae. I. Mast cells, basophils, and eosinophils. Gastroenterology. 1985;89:92–101
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  18. Sandle GI, McGlone F, Crowe P, Marsh MN. Sodium transport properties of colonic epithelium in patients with inflammatory bowel disease (abstr.). Gastroenterology. 1987;92:1613
  19. Doe WF, Henry K, Booth CC. Complement in coeliac disease. In:  Hekkens W,  Pena A editor. Coeliac disease. Leiden: Stenfert Kroese; 1975;p. 189–194
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  22. Dvorak H, Mihm MC, Dvorak AM, Johnson RA, Manseau J. Morphology of delayed-type hypersensitivity reactions in man. I—Quantitative description of the inflammatory response. Lab Invest. 1974;31:111–130
  23. Dvorak AM, Mihm MC, Dvorak H. Morphology of delayedtype hypersensitivity in man. II—Ultrastructural alterations affecting the microvasculature and the tissue mast cells. Lab Invest. 1976;34:179–191
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  26. Dobbins WO. Human intestinal intraepithelial lymphocytes. Gut. 1986;27:972–985
  27. Marsh MN. Functional and structural aspects of epithelial lymphocytes with implications for coeliac disease and tropical sprue. Scand J Gastroenterol. 1985;114:55–75 (Suppl)
  28. Freedman AR, Macartney JC, Nelifer JM, Ciclitira P. Timing of infiltration of T lymphocytes induced by gluten into the small intestine in coeliac disease. J Clin Pathol. 1987;40:741–745
  29. Ferguson A. Models of immunologically-driven small intestinal damage. In:  Marsh MN editors. Immunopathology of the small intestine. Chichester: Wiley; 1987;p. 225–252
  30. Mowat AM, Ferguson A. Intra-epithelial lymphocyte count and crypt hyperplasia measure the mucosal component of the graft-versus-host reaction in mouse small intestine. Gastroenterology. 1982;83:417–423
  31. Fry L, Seah PP, McMinn RMH, Hoffbrand AV. Lymphocytic infiltration of epithelium in diagnosis of gluten-sensitive enteropathy. Br Med J [Clin Res]. 1972;3:371–374
  32. Marsh MN, Bjarnason I, Shaw J, Ellis A, Baker R, Peters TJ. Studies of intestinal lymphoid tissue. XIV-HLA status, mucosal morphology, permeability and epithelial lymphocyte populations in first-degree relatives of patients with coeliac disease. Gut. 1989; (in press)
  33. Marsh MN. Studies of intestinal lymphoid tissue: XI—The immunopathology of cell-mediated reactions in gluten sensitivity and other enteropathies. Scanning Microsc. 1988;2:1663–1684
  34. Padykula HA, Strauss EW, Ladman AJ, Gardner FH. A morphologic and histochemical analysis of the human jejunal epithelium in non-tropical sprue. Gastroenterology. 1961;40:735–765
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  37. Loft D, Crowe P, Marsh MN. Rectal gluten challenge in the diagnosis of celiac sprue (CS): a prospective study (abstr.). Gastroenterology. 1988;94:268

☆ This work was presented at the 88th Annual Meeting of the American Gastroenterological Association, Chicago, 1987.

PII: 0016-5085(89)91411-X

Gastroenterology
Volume 97, Issue 1 , Pages 29-37 , July 1989

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