Gastroenterology
Volume 87, Issue 1 , Pages 28-36 , July 1984

Regional gastric mucosal blood flow measurements by hydrogen gas clearance in the anesthetized rat and rabbit

  • Felix W. Leung

      Affiliations

    • Corresponding Author InformationAddress requests for reprints to: Felix W. Leung, M.D., Veterans Administration Wadsworth Medical Center, 691/111C, Los Angeles, California 90073.
    • Center for Ulcer Research and Education (CURE), Research, Medical, and Surgical Services, Veterans Administration Wadsworth Medical Center Los Angeles, California, USA
    • UCLA School of Medicine, Los Angeles, California, USA
    • ,
  • Paul H. Guth

      Affiliations

    • Center for Ulcer Research and Education (CURE), Research, Medical, and Surgical Services, Veterans Administration Wadsworth Medical Center Los Angeles, California, USA
    • UCLA School of Medicine, Los Angeles, California, USA
    • ,
  • Oscar U. Scremin

      Affiliations

    • Center for Ulcer Research and Education (CURE), Research, Medical, and Surgical Services, Veterans Administration Wadsworth Medical Center Los Angeles, California, USA
    • UCLA School of Medicine, Los Angeles, California, USA
    • ,
  • Elizabeth M. Golanska

      Affiliations

    • Center for Ulcer Research and Education (CURE), Research, Medical, and Surgical Services, Veterans Administration Wadsworth Medical Center Los Angeles, California, USA
    • UCLA School of Medicine, Los Angeles, California, USA
    • ,
  • Gordon L. Kauffman Jr.

      Affiliations

    • Center for Ulcer Research and Education (CURE), Research, Medical, and Surgical Services, Veterans Administration Wadsworth Medical Center Los Angeles, California, USA
    • UCLA School of Medicine, Los Angeles, California, USA

Received 22 August 1983 ,Accepted 22 January 1984.

References 

  1. Guth PH, Baumann H, Grossman MI, et al.  Measurement of gastric mucosal blood flow in man. Gastroenterology. 1978;74:831–834
  2. Taylor TV, Pullan BR, Elder JB, et al.  Observations of gastric mucosal blood flow using 99mTc in rat and man. Br J Surg. 1975;62:788–791
  3. Knight SE, McIsaac RL. Neutral red clearance as an estimate of gastric mucosal blood flow in man. J Physiol. 1977;272:62P–63P
  4. Knight SE, McIsaac RL, Fielding LP. The effect of highly selective vagotomy on the relationship between gastric mucosal blood flow and acid secretion in man. Br J Surg. 1978;65:721–723
  5. Knight SE, McIsaac RL, Rennie CD. The effect of histamine and histamine antagonists on gastric acid secretion and mucosal blood flow in man. Br J Surg. 1980;67:266–268
  6. Kamada T, Sato N, Kawano S, et al.  Gastric mucosal hemodynamics after thermal or head injury. Gastroenterology. 1982;83:535–540
  7. Kamada T, Kawano S, Sato N, et al.  Gastric mucosal blood distribution and its changes in the healing process of gastric ulcer. Gastroenterology. 1983;84:1541–1546
  8. Archibald LH, Moody FG, Simons M. Measurement of gastric blood flow with radioactive microspheres. J Appl Physiol. 1975;38:1051–1056
  9. Wechsler RL, Dugas MC. Human G.I. mucosal blood flow (MBF) measured by the iodoantipyrine clearance technique (abstr). Gastroenterology. 1983;84:1346
  10. Murakami M, Moriga M, Miyake T, et al.  Contact electrode method in hydrogen gas clearance technique: a new method for determination of regional gastric mucosal blood flow in animals and humans. Gastroenterology. 1982;82:457–467
  11. Cheung LY, Sonnenschein LA. Measurements of regional gastric mucosal blood flow by hydrogen gas clearance. Am J Surg. 1984;147:32–37
  12. Kety SS. Theory of blood-tissue exchange and its application to measurement of blood flow. In:  Bruner HD editors. Methods in medical research VIII. Chicago: Year Book Publishers; 1960;p. 223–227
  13. Kety SS, Schmidt CF. The determination of cerebral blood flow in man by the use of nitrous oxide in low concentration. Am J Physiol. 1945;143:53–66
  14. Aukland K, Bower BF, Berliner RW. Measurement of local blood flow with hydrogen gas. Cir Res. 1964;14:164–187
  15. Fatt I. Diffusion at the polarographic oxygen sensor. In: Polarographic oxygen sensor—its theory of operation and its application in biology, medicine and technology. Cleveland: CRC Press; 1976;p. 9–17
  16. Kauffman GL. Blood flow and gastric secretion. In: 6th ed.. Fed Proc. 41:1982;p. 2080–2083
  17. Brodie BB, Axelrod J. The fate of aminopyrine (pyramidon) in man and methods for the estimation of aminopyrine and its metabolites in biological material. J Pharmacol Exp Ther. 1950;99:171–184
  18. Guth PH, Paulsen G. Prostaglandin cytoprotection does not involve interference with aspirin absorption. In: 6th ed.. Proc Soc Exp Biol Med. 162:1979;p. 128–130
  19. Halsey JH, Capra NF, McFarland RS. Use of hydrogen for measurement of regional cerebral blood flow: problem of intercompartmental diffusion. Stroke. 1977;8:351–357
  20. Miller GL, Ng KC. The hydrogen clearance technique in measuring steady-state and transient changes in local blood flow. In:  Garlick D editors. Progress in microcirculation research. Sydney: Committee in Postgraduate Med. Ed. UNSW; 1981;p. 413–428
  21. Becker H, Manganaro A, Lazar H, et al.  Limitations of studying splanchnic blood flow during anesthesia. In: 6th ed.. Surg Forum. 30:1976;p. 347–349
  22. Shirazi SS, Mueller TM, Hardy BM. Canine gastric acid secretion and blood flow measurement in hemorrhagic shock. Gastroenterology. 1977;73:75–78
  23. Polansky DB, Shirazi SS, Coon D. Lack of correlation of gastric acid secretion and blood flow. J Surg Res. 1979;26:320–325
  24. Delaney JP, Michel HM, Bond J. Cimetidine and gastric blood flow. Surgery. 1978;84:190–193
  25. Rudick I, Werther JL, Chapman ML, et al.  Mucosal blood flow in canine antral and fundic pouches. Gastroenterology. 1971;60:263–271
  26. Semb BKH. Gastric flow measured with hydrogen clearance technique. Scand J Gastroenterol. 1979;14:641–646
  27. Semb BKH. Regional gastric flow determined by a hydrogen clearance technique in anesthetized and conscious animals. Scand J Gastroenterol. 1980;15:569–576
  28. Semb BKH. Changes of regional gastric flow measured by hydrogen clearance techniques after histamine stimulation in conscious animals. Scand J Gastroenterol. 1981;16:795–800
  29. Skarstein A, Svanes K, Soreide O, et al.  Effect of pentagastrin on blood flow distribution in the stomach of cats with gastric ulcer. Scand J Gastroenterol. 1977;12:71–76
  30. Taylor TV, Pullan BR, Goddard J, et al.  Effect of secretagogues on mucosal blood flow in the antrum and corpus of the stomach. Gut. 1978;19:14–18
  31. Guth PH, Smith E. The effect of gastrointestinal hormones on the gastric microcirculation. Gastroenterology. 1976;71:435–438
  32. Bell PRF, Shelley T. Gastric mucosal blood flow and acid secretion in conscious animals measured by heat clearance. Am J Dig Dis. 1968;13:685–696
  33. Skarstein A. Effect of vasopressin on blood flow distribution in the stomach of cats with gastric ulcer. Scand J Gastroenterol. 1978;13:783–788
  34. Jacobson ED, Linford RH, Grossman MI. Gastric secretion in relation to mucosal blood flow studied by a clearance technic. J Clin Invest. 1966;45:1–13
  35. Delaney JP, Grim E. Experimentally induced variations in canine gastric blood flow and its distribution. Am J Physiol. 1965;208:353–358
  36. Cheung LY. Topical effects of 16,16-dimethyl prostaglandin E2 on gastric blood flow in dogs. Am J Physiol. 1980;238:G514–G519
  37. Sonnenberg A, Blum AL. Limitations to measurement of gastric mucosal blood flow by 14C-aminopyrine clearance. In:  Fielding LP editors. Gastrointestinal mucosal blood flow. Edinburgh: Churchill Livingstone; 1980;p. 43–58
  38. Muller-Lissner SA, Sonnenberg A, Blum AL. Does gastric aminopyrine clearance reflect gastric mucosal blood flow or parietal cell function?. Gut. 1981;23:997–1002

☆ This work was supported by National Institute of Arthritis, Metabolism, and Digestive Diseases Grants AM 25891, 27465, and 17328 to the Center for Ulcer Research and Education (CURE) and by Veterans Administration Research funds.

PII: 0016-5085(84)90122-7

Gastroenterology
Volume 87, Issue 1 , Pages 28-36 , July 1984

Article Tools